Herrmann J L, Bakoss P, Korver H, Bulu A A, Bellenger E, Terpstra W J, Saint Girons I, Baranton G
Unité de Bactériologie Moléculaire et Médicale, Institut Pasteur, Paris, France.
Int J Syst Bacteriol. 1994 Apr;44(2):362-4. doi: 10.1099/00207713-44-2-362.
Pulsed-field gel electrophoresis (PFGE) studies performed with leptospiral isolates led us to suspect the existence of a new serovar in the Grippotyphosa serogroup. The results obtained with reference serological techniques used in leptospiral identification, including cross-agglutination absorption and monoclonal antibody techniques, confirmed the existence of a new serovar exemplified by strain Dadas I. Four other isolates from different regions of the world were submitted for identification by PFGE and produced NotI restriction patterns similar to that of strain Dadas I. Our data demonstrate the power of PFGE for identifying leptospiral isolates. The name dadas is proposed for the new serovar.
对钩端螺旋体分离株进行的脉冲场凝胶电泳（PFGE）研究使我们怀疑在波摩那群血清型中存在一种新的血清型。使用钩端螺旋体鉴定中使用的参考血清学技术（包括交叉凝集吸收和单克隆抗体技术）获得的结果证实了以达达一号菌株为例的新血清型的存在。来自世界不同地区的其他四个分离株被提交进行PFGE鉴定，并产生了与达达一号菌株相似的NotI限制性酶切图谱。我们的数据证明了PFGE在鉴定钩端螺旋体分离株方面的强大作用。建议将“达达”作为新血清型的名称。
