Ota A, Ichinose H, Kobayashi K, Morita S, Sawada H, Mizuguchi T, Nagatsu T
Joint Research Division for Therapies against Intractable Diseases, School of Medicine, Fujita Health University, Aichi, Japan.
Neurosci Lett. 1994 Jan 17;166(1):55-8. doi: 10.1016/0304-3940(94)90839-7.
We investigated the effect of subcutaneous injection of nicotine on in vitro tyrosine hydroxylase (TH) activity in adrenal gland and brain of the transgenic mice carrying an 11-kb fragment containing the entire human TH gene. Injection of 5 mg nicotine/kg (as free base) for 3 days caused a statistically significant increase in vitro TH activity in the adrenal gland, whereas brain TH activity was not affected at all. The adrenal gland of non-transgenic C57BL/6J mice treated in the same way as for transgenic mice tended to enhance TH activity, although not to a significant level. This observation might indicate the possibility that the machinery used by nicotine in regulating the properties or expression of TH in the adrenal gland should be similar between transgenic and non-transgenic mice.
我们研究了皮下注射尼古丁对携带包含整个人类酪氨酸羟化酶（TH）基因的11 kb片段的转基因小鼠肾上腺和大脑中TH体外活性的影响。以5 mg尼古丁/千克（以游离碱计）注射3天导致肾上腺中TH体外活性出现统计学上的显著增加，而大脑中的TH活性则完全未受影响。以与转基因小鼠相同的方式处理的非转基因C57BL/6J小鼠的肾上腺，其TH活性虽未达到显著水平，但有增强的趋势。这一观察结果可能表明，在调节肾上腺中TH的特性或表达方面，尼古丁所利用的机制在转基因小鼠和非转基因小鼠之间应该是相似的。
