Norman A B, Khosla U M, Klug J M, Thompson H R
Department of Psychiatry, University of Cincinnati College of Medicine, OH 45267-0559.
Brain Res. 1994 Feb 28;638(1-2):352-6. doi: 10.1016/0006-8993(94)90671-8.
Autoradiographic measurements of peroxidase labelled tyrosine hydroxylase immunoreactivity in brain sections from rats with unilateral 6-hydroxydopamine lesions were obtained using enhanced chemiluminescence. The autoradiograms required only 5-60 s exposure and displayed a good signal-to-noise ratio. The autoradiograms were quantitated using densitometry and by comparison with a standard curve. Following acquisition of the autoradiograms the sections were stained with the chromogen diaminobenzidine (DAB). Distribution of the DAB closely followed the grain densities observed in the autoradiograms on both the lesioned and unlesioned side of the brain. This method represents a simple, rapid and inexpensive technique for performing both quantitative autoradiography and cytochemical studies in individual tissue sections of any peroxidase labelled immunoreactivity.
使用增强化学发光法对单侧6-羟基多巴胺损伤大鼠脑切片中过氧化物酶标记的酪氨酸羟化酶免疫反应性进行放射自显影测量。放射自显影片只需曝光5 - 60秒，且具有良好的信噪比。使用光密度测定法并与标准曲线比较对放射自显影片进行定量分析。获取放射自显影片后，将切片用显色剂二氨基联苯胺（DAB）染色。DAB的分布与在大脑损伤侧和未损伤侧放射自显影片中观察到的颗粒密度密切相关。该方法代表了一种简单、快速且廉价的技术，可用于在任何过氧化物酶标记免疫反应性的单个组织切片中进行定量放射自显影和细胞化学研究。
