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潜在水污染物和酶抑制剂对大肠杆菌自动快速检测的影响。

Effect of potential water pollutants and enzyme inhibitors on an automated rapid test for Escherichia coli.

作者信息

Moran J W, Smith T L, Witter L D

出版信息

Appl Environ Microbiol. 1976 Oct;32(4):645-6. doi: 10.1128/aem.32.4.645-646.1976.

DOI:10.1128/aem.32.4.645-646.1976
PMID:791128
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC170327/
Abstract

The effect of selected potential inhibitors that may be found in water or wastewater on the activity of glutamate decarboxylase (EC 4.1.1.5) from Escherichia coli was determined. Several classes of compounds inhibited the enzyme, but those expected to be most frequently encountered were the heavy metal ions, the phosphates, and possibly the sulfates. From the results, it was judged that these compounds should not adversely affect the routine usage of this enzyme in an automated rapid test for E. coli.

摘要

测定了可能存在于水或废水中的某些潜在抑制剂对大肠杆菌谷氨酸脱羧酶(EC 4.1.1.5)活性的影响。几类化合物会抑制该酶,但预计最常遇到的是重金属离子、磷酸盐,可能还有硫酸盐。从结果判断,这些化合物不应在大肠杆菌的自动快速检测中对该酶的常规使用产生不利影响。

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本文引用的文献

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Studies on bacterial amino-acid decarboxylases: 6. Codecarboxylase content and action of inhibitors.细菌氨基酸脱羧酶的研究:6. 辅酶含量及抑制剂的作用
Biochem J. 1945;39(1):52-8. doi: 10.1042/bj0390052.
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
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Glutamic acid decarboxylase. I. Isolation procedures and properties of the enzyme.谷氨酸脱羧酶。I. 酶的分离方法及性质
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4
Structure of the binding site of pyridoxal 5'-phosphate to Escherichia coli glutamate decarboxylase.磷酸吡哆醛与大肠杆菌谷氨酸脱羧酶结合位点的结构
Biochemistry. 1970 Jan 20;9(2):233-8. doi: 10.1021/bi00804a007.
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Chemical and physical properties of Escherichia coli glutamate decarboxylase.大肠杆菌谷氨酸脱羧酶的化学和物理性质。
Biochemistry. 1970 Jan 20;9(2):226-33. doi: 10.1021/bi00804a006.
6
Coenzyme analog inhibitors of apoglutamate decarboxylase.脱辅基谷氨酸脱羧酶的辅酶类似物抑制剂
Biochemistry. 1969 Mar;8(3):1117-22. doi: 10.1021/bi00831a044.
7
Kinetics and mechanism of the binding of pyridoxal 5'-phosphate to apoglutamate decarboxylase. Evidence for a rate-determining conformation change.
J Biol Chem. 1972 Nov 10;247(21):7097-105.
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Glutamate decarboxylase. Substrate specificity and inhibition by carboxylic acids.
Biochemistry. 1972 Mar 28;11(7):1304-9. doi: 10.1021/bi00757a029.
9
Formation of methyl mercury by bacteria.细菌形成甲基汞。
Appl Microbiol. 1975 Sep;30(3):424-32. doi: 10.1128/am.30.3.424-432.1975.