Moran J W, Smith T L, Witter L D
Appl Environ Microbiol. 1976 Oct;32(4):645-6. doi: 10.1128/aem.32.4.645-646.1976.
The effect of selected potential inhibitors that may be found in water or wastewater on the activity of glutamate decarboxylase (EC 4.1.1.5) from Escherichia coli was determined. Several classes of compounds inhibited the enzyme, but those expected to be most frequently encountered were the heavy metal ions, the phosphates, and possibly the sulfates. From the results, it was judged that these compounds should not adversely affect the routine usage of this enzyme in an automated rapid test for E. coli.
测定了可能存在于水或废水中的某些潜在抑制剂对大肠杆菌谷氨酸脱羧酶(EC 4.1.1.5)活性的影响。几类化合物会抑制该酶,但预计最常遇到的是重金属离子、磷酸盐,可能还有硫酸盐。从结果判断,这些化合物不应在大肠杆菌的自动快速检测中对该酶的常规使用产生不利影响。