Milne D S, Gascoigne A D, Wilkes J, Sviland L, Ashcroft T, Malcolm A J, Corris P A
University Department of Pathology, Royal Victoria Infirmary, Newcastle-upon-Tyne, United Kingdom.
Transplantation. 1994 Jun 27;57(12):1762-6.
The expression of MHC class II antigens and ICAM-1 and the composition of lymphocyte infiltrates have been studied in frozen sections of transbronchial biopsies from lung transplant recipients. First, biopsies obtained from patients who showed acute rejection, OB, and normal features were compared. Second, we compared first-year biopsies from patients developing OB and patients with a good clinical outcome. HLA-DR was widely expressed on epithelia and vascular endothelium. Increased vascular HLA-DP expression was found in OB biopsies. In OB patients there was a significantly increased frequency of bronchial HLA-DP and vascular HLA-DQ expression. Expression of ICAM-1 by bronchial and bronchiolar basal cells, a phenomenon not reported previously in humans, was seen in a small number of biopsies. CD8 predominant lymphocytic infiltrates were present in all groups and were increased in OB biopsies and OB patients. Increased numbers of CD4-positive cells were found in rejection and OB when compared with normal biopsies. These findings support an immunological basis for the development of OB.
对肺移植受者经支气管活检的冰冻切片中MHC II类抗原和细胞间黏附分子-1（ICAM-1）的表达以及淋巴细胞浸润的组成进行了研究。首先，比较了取自表现出急性排斥反应、闭塞性细支气管炎（OB）和正常特征患者的活检样本。其次，我们比较了发生OB的患者和临床结局良好的患者的第一年活检样本。HLA-DR在上皮细胞和血管内皮细胞上广泛表达。在OB活检样本中发现血管HLA-DP表达增加。在OB患者中，支气管HLA-DP和血管HLA-DQ表达频率显著增加。在少数活检样本中可见支气管和细支气管基底细胞表达ICAM-1，这一现象此前在人类中未见报道。所有组均存在以CD8为主的淋巴细胞浸润，在OB活检样本和OB患者中浸润增加。与正常活检样本相比，在排斥反应和OB中发现CD4阳性细胞数量增加。这些发现支持了OB发生的免疫学基础。
