Deméocq F, Kanold J, Chassagne J, Bezou M J, Lutz P, deLumley L, Philip I, Vannier J P, Margueritte G, Lamagnére J P
Departement de Pediatrie, Hotel-Dieu, Clermont-Ferrand, France.
Bone Marrow Transplant. 1994 Jan;13(1):43-50.
Peripheral blood stem cells (PBSCs) were collected for autotransplantation in 20 children (median age 4 years, range 0.5-10 years) weighing < 25 kg (median 14.5 kg, range 6.8-24 kg) with various malignant diseases: leukemias and lymphomas (n = 6), solid tumours (n = 14). Cytaphereses were carried out after standard chemotherapy (n = 10), mobilizing high-dose chemotherapy (n = 9) or radiotherapy alone (n = 1). In 13 children PBSCs were harvested after haematopoietic growth factor (HGF) administration. PBSCs were collected using a continuous flow blood separator (Cobe Spectra). In 13 patients access was through a central catheter with peripheral venous return, in 4 patients access was through a central catheter with return through a femoral catheter; one patient had femoral catheter access with peripheral venous return and two patients had both access and return through peripheral veins. For 19 patients the extracorporeal line was primed with red blood cells. The median blood flow rate was 13.8 ml/min (range 7-22 ml/min). Sixty-six procedures (mean 3.3/patient, range 1-4) were performed with a mean total collection time of 8.5 h. The median number of granulocyte-macrophage colony-forming units (CFU-GM) collected was 37.7 x 10(4)/kg (mean 107 x 10(4)/kg, range 1.05-882 x 10(4)/kg). The number of CFU-GM collected per procedure in children with HGF was 7.8-fold higher than in children without HGF (median 20.4 versus 2.6 x 10(4) CFU-GM/kg body weight, respectively). There were no consistent effects on peripheral blood counts except on platelet counts which decreased following each procedure (median decrease in platelet count was 36%).(ABSTRACT TRUNCATED AT 250 WORDS)
对20名患有各种恶性疾病的儿童（年龄中位数4岁，范围0.5 - 10岁，体重<25 kg，中位数14.5 kg，范围6.8 - 24 kg）采集外周血干细胞（PBSCs）用于自体移植，这些疾病包括白血病和淋巴瘤（n = 6）、实体瘤（n = 14）。在标准化疗后进行血细胞分离术（n = 10）、动员性大剂量化疗后（n = 9）或单独放疗后（n = 1）进行血细胞分离术。13名儿童在给予造血生长因子（HGF）后采集PBSCs。使用连续流动血液分离器（Cobe Spectra）采集PBSCs。13例患者通过中心静脉导管外周静脉回血进行采集，4例患者通过中心静脉导管股静脉回血进行采集；1例患者通过股静脉导管外周静脉回血进行采集，2例患者通过外周静脉进出血。19例患者的体外循环管路用红细胞预充。中位血流速度为13.8 ml/分钟（范围7 - 22 ml/分钟）。共进行了66次操作（平均每位患者3.3次，范围1 - 4次），平均总采集时间为8.5小时。采集的粒细胞 - 巨噬细胞集落形成单位（CFU - GM）中位数为37.7×10⁴/kg（平均107×10⁴/kg，范围1.05 - 882×10⁴/kg）。接受HGF的儿童每次操作采集的CFU - GM数量比未接受HGF的儿童高7.8倍（中位数分别为20.4和2.6×10⁴CFU - GM/kg体重）。除每次操作后血小板计数下降（血小板计数中位数下降36%）外，对外周血细胞计数没有一致的影响。（摘要截短于250字）
