Kvam E, Stokke T
Department of Biophysics, Norwegian Radium Hospital, Montebello, Oslo, Norway.
Photochem Photobiol. 1994 Apr;59(4):437-40. doi: 10.1111/j.1751-1097.1994.tb05061.x.
Human REH cells were incubated with the photosensitizers meso-tetra(4-sulfonatophenyl)porphyrin (TSPP = TPPS4) or meso-tetra(3-hydroxyphenyl)porphyrin (3-THPP). The relatively hydrophilic TSPP was partly found in the cytoplasm and partly in the nuclei, whereas the lipophilic 3-THPP was found apparently in membranes and not inside the nuclei. After illumination, sites of DNA repair were labeled by means of a monoclonal antibody against proliferating cell nuclear antigen (PCNA) bound in the nuclei. The amount of bound PCNA in non-S-phase cells was proportional to the light dose. The bound PCNA was homogeneously distributed in the nuclei 0.5 h after photodynamic treatment (PDT) with TSPP. In contrast, for cells given PDT with 3-THPP, the periphery of the nuclei was selectively labeled, indicating that the initial DNA damage was localized close to the sensitizer at the nuclear membrane.
将人REH细胞与光敏剂中-四(4-磺酸苯基)卟啉(TSPP = TPPS4)或中-四(3-羟基苯基)卟啉(3-THPP)一起孵育。相对亲水的TSPP部分存在于细胞质中，部分存在于细胞核中，而亲脂性的3-THPP明显存在于细胞膜中，而非细胞核内。光照后，通过结合在细胞核中的抗增殖细胞核抗原(PCNA)单克隆抗体标记DNA修复位点。非S期细胞中结合的PCNA量与光剂量成正比。用TSPP进行光动力治疗(PDT) 0.5小时后，结合的PCNA在细胞核中均匀分布。相比之下，对于用3-THPP进行PDT的细胞，细胞核周边被选择性标记，表明初始DNA损伤位于核膜处靠近敏化剂的位置。
