Young N T, Darke C
Regional Tissue Typing Laboratory National Blood Transfusion Service, Wales, Cardiff, UK.
Tissue Antigens. 1994 Jan;43(1):28-33. doi: 10.1111/j.1399-0039.1994.tb02292.x.
Molecular studies of HLA-DRB, -DRA and -DQB1 genes in the variant DR2 haplotype, DR2LUM, were performed using the homozygous lymphoblastoid cell line, CTS. The results of HLA Class II gene RFLP and PCR analyses suggest that DR2LUM was created by a homologous recombination event between HLA-DR1 and HLA-DR15 haplotypes. Evidence for the presence of a recombinational "hotspot" in haplotypes possessing a DRB6 pseudogene is presented. The results of this study have important implications for detection of HLA-DR2 alleles in DRB gene oligotyping strategies, and suggest that the CTS cell line will be a useful addition to cell panels for characterizing HLA antisera.
利用纯合淋巴母细胞系CTS对变异DR2单倍型DR2LUM中的HLA - DRB、- DRA和 - DQB1基因进行了分子研究。HLA II类基因RFLP和PCR分析结果表明，DR2LUM是由HLA - DR1和HLA - DR15单倍型之间的同源重组事件产生的。文中提供了在含有DRB6假基因的单倍型中存在重组“热点”的证据。本研究结果对DRB基因寡核苷酸分型策略中HLA - DR2等位基因的检测具有重要意义，并表明CTS细胞系将是用于鉴定HLA抗血清的细胞面板中的一个有用补充。
