Priest F G, Kaji D A, Rosato Y B, Canhos V P
Department of Biological Sciences, Heriot-Watt University, Riccarton, Edinburgh, UK.
Microbiology (Reading). 1994 May;140 ( Pt 5):1015-22. doi: 10.1099/13500872-140-5-1015.
Ribosomal RNA gene restriction patterns have been determined for 43 strains of Bacillus thuringiensis representing 10 serovars and eight reference strains of B. anthracis, B. cereus and B. mycoides. Strains within a B. thuringiensis serovar produced highly related or identical ribotype patterns: in particular, 12 strains of serovar israelensis, five strains of serovar kurstaki, two strains of serovar galleriae and three strains of serovar aizawa produced ribotype patterns consistent with serotype designations. Moreover, variety tenebrionis (serotype 8a8b), a coleopteran pathogen, could be distinguished from the more common lepidopteran pathogens of this serotype (serovar morrisoni) by ribotyping. The correlation of ribotype patterns with serotype suggests a clonal population structure for B. thuringiensis.
已确定了43株苏云金芽孢杆菌的核糖体RNA基因限制模式，这些菌株代表10个血清型，还包括炭疽芽孢杆菌、蜡样芽孢杆菌和蕈状芽孢杆菌的8个参考菌株。苏云金芽孢杆菌血清型内的菌株产生高度相关或相同的核糖体分型模式：特别是，12株以色列血清型菌株、5株库尔斯塔克血清型菌株、2株蜡螟血清型菌株和3株 aizawa血清型菌株产生的核糖体分型模式与血清型命名一致。此外，鞘翅目病原菌暗黑变种（血清型8a8b）可通过核糖体分型与该血清型更常见的鳞翅目病原菌（莫氏血清型）区分开来。核糖体分型模式与血清型的相关性表明苏云金芽孢杆菌具有克隆群体结构。
