独特的T细胞分化标志物：对小鼠胎儿胸腺细胞具有霍乱毒素受体活性的神经节苷脂。

Unique T cell differentiation markers: gangliosides with cholera toxin receptor activity on murine fetal thymocytes.

作者信息

Noguchi M, Suping Z, Taguchi J, Hirano T, Hashimoto H, Hirose S, Iwamori M, Okumura K

机构信息

Department of Rheumatology, Juntendo University School of Medicine, Tokyo, Japan.

出版信息

Cell Immunol. 1994 Jul;156(2):402-13. doi: 10.1006/cimm.1994.1185.

DOI:10.1006/cimm.1994.1185

PMID:7912995

Abstract

Cholera toxin B subunit receptors (CTBR; gangliosides GM1a and GM1b-GalNAc-Gal) and GM1b-type gangliosides were examined during T cell development in BALB/c mice by FACS or TLC immunostaining. Sixty-two percent of CD4-8- early fetal thymocytes express CTBR [58% low affinity (GM1a+), 4% high affinity (GM1a2+)] at 13 days gestation (Day 13); GM1a2+ was expressed preferentially on Thy-1+ fetal thymocytes after Day 13. GM1a2+ Thy-1+ cells increased from 4% on Day 13 to 95% on Day 17. Surface GM1a2+ decreased beginning on Day 15 while GM1b-GalNAc-Gal (and CD4 and CD8) increased. GM1b-type+ cells increased from 8% on Day 13 to 51% on Day 17 and then decreased to 16% at 4 weeks; few asialo GM1+ cells (< 5%) are seen during gestation. Thus, synthesis of GM1a- and GM1b-type series was active until Days 15 and 17, respectively, and then was suppressed; GM1b-GalNAc-Gal was synthesized from GM1b following Gestational Day 15. GM1a- and GM1b-type identify immature T cells in mice, while GM1b-GalNAc-Gal identifies mature T cells.

摘要

通过荧光激活细胞分选术（FACS）或薄层层析免疫染色法，在BALB/c小鼠的T细胞发育过程中检测霍乱毒素B亚基受体（CTBR；神经节苷脂GM1a和GM1b-GalNAc-Gal）和GM1b型神经节苷脂。妊娠13天（第13天）时，62%的CD4-8-早期胎儿胸腺细胞表达CTBR [58%为低亲和力（GM1a+），4%为高亲和力（GM1a2+）]；第13天后，GM1a2+优先在Thy-1+胎儿胸腺细胞上表达。GM1a2+ Thy-1+细胞从第13天的4%增加到第17天的95%。从第15天开始，表面GM1a2+减少，而GM1b-GalNAc-Gal（以及CD4和CD8）增加。GM1b型+细胞从第13天的8%增加到第17天的51%，然后在4周时降至1%；妊娠期间几乎看不到脱唾液酸GM1+细胞（<5%）。因此，GM1a型和GM1b型系列的合成分别在第15天和第17天之前活跃，然后受到抑制；妊娠第15天后，GM1b-GalNAc-Gal由GM1b合成。GM1a型和GM1b型可识别小鼠中的未成熟T细胞，而GM1b-GalNAc-Gal可识别成熟T细胞。