人类c-erbB-2启动子中类似起始子元件和TATA盒对两个位点转录起始的独立控制。

Independent control of transcription initiations from two sites by an initiator-like element and TATA box in the human c-erbB-2 promoter.

作者信息

Mizuguchi G, Kanei-Ishii C, Sawazaki T, Horikoshi M, Roeder R G, Yamamoto T, Ishii S

机构信息

Laboratory of Molecular Genetics, Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.

出版信息

FEBS Lett. 1994 Jul 4;348(1):80-8. doi: 10.1016/0014-5793(94)00578-8.

DOI:10.1016/0014-5793(94)00578-8

PMID:7913049

Abstract

Transcription of the human c-erbB-2-proto-oncogene starts mainly at two sites, nucleotide positions +1 and -69. The present studies have identified an initiator-like element that specifies the position of transcription initiation at position -69. This initiator-like element contains six GGA repeats and is located just downstream from the transcription start site between positions -68 and -45. In addition, both in vitro and in vivo studies indicated that transcription initiation at position +1 is specified by a TATA box 25 bp upstream from the transcription startpoint. Thus, initiation at two sites in the c-erbB-2 promoter is controlled independently by the initiator-like element and the TATA box.

摘要

人类c-erbB-2原癌基因的转录主要起始于两个位点，核苷酸位置+1和-69。目前的研究已经鉴定出一种类似起始子的元件，它指定了转录起始于-69位置。这种类似起始子的元件包含六个GGA重复序列，位于转录起始位点下游-68至-45位置之间。此外，体外和体内研究均表明，+1位置的转录起始由转录起始点上游25 bp处的TATA盒指定。因此，c-erbB-2启动子中两个位点的起始由类似起始子的元件和TATA盒独立控制。