天蓝色链霉菌A3(2)中两个groEL基因的特性分析

Characterization of two groEL genes in Streptomyces coelicolor A3(2).

作者信息

Duchêne A M, Kieser H M, Hopwood D A, Thompson C J, Mazodier P

机构信息

Unité de Génie Microbiologique, Institut Pasteur, Paris, France.

出版信息

Gene. 1994 Jun 24;144(1):97-101. doi: 10.1016/0378-1119(94)90210-0.

DOI:10.1016/0378-1119(94)90210-0

PMID:7913076

Abstract

Two Streptomyces coelicolor A3(2) groEL-like genes, groEL1 and groEL2, were cloned and characterized. Pulsed-field-gel electrophoresis located these genes, which were not adjacent, in the same segment of the chromosome. Nucleotide sequence analysis revealed that groEL1, but not groEL2, was preceded by a groES-like gene. Northern blots showed that heat shock induced the accumulation of transcripts corresponding to groES (0.7 kb), groES/EL1 (2.3 kb) and groEL2 (2.1 kb). Unique transcription start points and promoters were located for groES/EL1 and groEL2, having -10 and -35 hexamers similar to eubacterial vegetative promoters. Regions located 5' to the groES/EL1 or groEL2 structural genes contain 'GCACTCN9GAGTGC' motifs conserved upstream from the heat-shock genes of other bacteria.

摘要

克隆并鉴定了天蓝色链霉菌A3(2)的两个类groEL基因groEL1和groEL2。脉冲场凝胶电泳将这两个不相邻的基因定位在染色体的同一段上。核苷酸序列分析表明，groEL1之前有一个类groES基因，而groEL2之前没有。Northern杂交显示，热休克诱导了与groES（0.7 kb）、groES/EL1（2.3 kb）和groEL2（2.1 kb）相对应的转录本的积累。确定了groES/EL1和groEL2独特的转录起始点和启动子，其-10和-35六聚体与真细菌营养型启动子相似。位于groES/EL1或groEL2结构基因5'端的区域含有“GCACTCN9GAGTGC”基序，该基序在其他细菌的热休克基因上游保守。