Gilboa E, Smith C
Department of Surgery, Duke University Medical Center, Durham, NC 27710.
Trends Genet. 1994 Apr;10(4):139-44. doi: 10.1016/0168-9525(94)90216-x.
Genetic manipulation of somatic cells may be of therapeutic value in a variety of infectious diseases, particularly in human immunodeficiency virus (HIV) infection. Stable insertion of custom-designed 'resistance genes' into cells susceptible to HIV could reduce the viral burden in infected individuals and potentially retard the characteristic progressive immune dysfunction. Alternatively, ectopic expression of genes that encode viral antigens might induce potent antiviral immune responses and form the basis for novel prophylactic and therapeutic vaccines. While laboratory studies have proved that the approach works in principle, preclinical and clinical studies will be necessary to evaluate the therapeutic benefit of such gene-based therapies.
对体细胞进行基因操作在多种传染病治疗中可能具有重要价值，尤其是在人类免疫缺陷病毒（HIV）感染方面。将定制设计的“抗性基因”稳定插入易受HIV感染的细胞中，可降低感染者体内的病毒载量，并有可能延缓典型的进行性免疫功能障碍。另外，编码病毒抗原的基因的异位表达可能会诱导强大的抗病毒免疫反应，并构成新型预防性和治疗性疫苗的基础。虽然实验室研究已证明该方法原则上可行，但仍需要进行临床前和临床研究来评估此类基因疗法的治疗效果。
