Yamada M, Hatanaka H
Division of Protein Biosynthesis, Osaka University, Japan.
Brain Res. 1994 Apr 18;643(1-2):173-80. doi: 10.1016/0006-8993(94)90023-x.
We examined the effect of interleukin-6 (human recombinant) on glutamate-induced neuronal death of cultured 20-day fetal rat hippocampal neurons. After 7 days in culture, the hippocampal neurons were markedly degenerated by the addition of L-glutamate and also N-methyl-D-aspartate. The neuronal death was prevented by the addition of MK801, a potent N-methyl-D-aspartate antagonist. Interleukin-6 at the concentration of 50 ng/ml has a significant preventive effect on the glutamate-induced neuronal death. Basic fibroblast growth factor at the concentration of 100 ng/ml gave also significant protective effect on hippocampal neurons, but nerve growth factor was ineffective in preventing the toxicity. It has been postulated that glutamate plays an important role in the pathogenesis of neuronal death such as ischemia and the various neurological diseases. Interleukin-6 might have somewhat physiological or pathological role in these events.
我们研究了白细胞介素-6（重组人）对谷氨酸诱导的原代培养20天胎鼠海马神经元死亡的影响。培养7天后，添加L-谷氨酸和N-甲基-D-天冬氨酸可使海马神经元明显退化。添加强效N-甲基-D-天冬氨酸拮抗剂MK801可防止神经元死亡。浓度为50 ng/ml的白细胞介素-6对谷氨酸诱导的神经元死亡具有显著的预防作用。浓度为100 ng/ml的碱性成纤维细胞生长因子对海马神经元也有显著的保护作用，但神经生长因子在预防毒性方面无效。据推测，谷氨酸在缺血和各种神经疾病等神经元死亡的发病机制中起重要作用。白细胞介素-6可能在这些事件中具有一定的生理或病理作用。
