Apodaca G, Enrich C, Mostov K E
Department of Anatomy, University of California, San Francisco 94143.
J Biol Chem. 1994 Jul 22;269(29):19005-13.
The effect of the calmodulin antagonist N-(4-aminobutyl)-5-chloro-2-naphthalenesulfonamide (W-13) on the endosomal system of Madin-Darby canine kidney cells was assessed. W-13 inhibited receptor-mediated IgA transcytosis, recycling of transferrin, and caused all material endocytosed from both surfaces of the cell to be delivered to exceptionally large, novel endosomal structures, which appear to be derived from early endosomes. Treatment with other calmodulin antagonists similarly inhibited transcytosis and caused large endosomes to form. These observations raise the possibility that calmodulin may be involved in regulating membrane trafficking through the endosomes of polarized epithelial cells.
评估了钙调蛋白拮抗剂N-(4-氨基丁基)-5-氯-2-萘磺酰胺（W-13）对犬肾Madin-Darby细胞内体系统的影响。W-13抑制受体介导的IgA转胞吞作用、转铁蛋白的循环利用，并使从细胞两面内吞的所有物质都被转运至异常大的新型内体结构，这些结构似乎源自早期内体。用其他钙调蛋白拮抗剂处理同样会抑制转胞吞作用并导致大的内体形成。这些观察结果提示，钙调蛋白可能参与调节极化上皮细胞内体的膜转运。
