Muller H K, Bucana C D, Kripke M L, Cox P A, Saijo S, Strickland F M
Department of Pathology, University of Tasmania, Hobart, Australia.
Cell Immunol. 1994 Aug;157(1):263-76. doi: 10.1006/cimm.1994.1221.
Exposure of murine skin to suberythemal doses of ultraviolet-B (UVB) radiation before contact sensitization alters the activity of antigen-presenting cells (APC) in the draining lymph nodes (DLN), decreases the contact hypersensitivity (CHS) response, and induces hapten-specific Ts cells. We determined whether in vivo UVB irradiation alters the ability of hapten-bearing APC from the DLN to form clusters with hapten-specific T lymphocytes. When APC from UV-irradiated, fluorescein isothiocyanate (FITC)-sensitized mice were mixed with a FITC-specific T-cell line, significantly fewer clusters formed compared with FITC+ APC from unirradiated mice. A higher percentage of clusters formed with APC from UV-irradiated mice were Mac-1+ and bound both CD4+ and CD8+ T cells, whereas FITC+ APC from nonirradiated mice bound CD4+ cells. These results suggest that UVB irradiation interferes with the induction of CHS by altering the functional interaction between APC and T cells, perhaps by altering the population of APC in the skin.
在接触致敏前，将小鼠皮肤暴露于亚红斑剂量的紫外线B（UVB）辐射下，会改变引流淋巴结（DLN）中抗原呈递细胞（APC）的活性，降低接触性超敏反应（CHS），并诱导半抗原特异性Ts细胞。我们确定了体内UVB照射是否会改变来自DLN的携带半抗原的APC与半抗原特异性T淋巴细胞形成簇的能力。当将来自紫外线照射、异硫氰酸荧光素（FITC）致敏小鼠的APC与FITC特异性T细胞系混合时，与来自未照射小鼠的FITC+APC相比，形成的簇明显更少。来自紫外线照射小鼠的APC形成的簇中，更高比例的簇是Mac-1+，并且结合了CD4+和CD8+T细胞，而来自未照射小鼠的FITC+APC结合CD4+细胞。这些结果表明，UVB照射可能通过改变皮肤中APC的群体，改变APC与T细胞之间的功能相互作用，从而干扰CHS的诱导。
