13号染色体区域13q14 - q21的缺失杂交断点图将19个遗传标记排列在10个区间内。

A deletion hybrid breakpoint map of the chromosomal region 13q14-q21 orders 19 genetic markers in 10 intervals.

作者信息

Kooy R F, Verlind E, Houwen R H, Shapiro D N, Hawthorn L A, Cowell J K, Scheffer H, Buys C H

机构信息

Department of Medical Genetics, University of Groningen, The Netherlands.

出版信息

Eur J Hum Genet. 1994;2(1):59-65. doi: 10.1159/000472342.

DOI:10.1159/000472342

PMID:7913868

Abstract

A deletion hybrid breakpoint map of the chromosomal region 13q14-q21 has been constructed using 19 DNA markers and 13 cell lines with breakpoints in this chromosomal region. The cell lines define 10 distinct intervals in this region, which spans approximately 20 Mb. The markers include 6 RFLP markers, 11 microsatellites that provisionally had been mapped to the region 13q14-21, and 2 new polymorphic CA-repeats that were developed from an EMBL3 library of cell line ICD, containing 13pter-q14.3. The following order of markers was established: CEN-D13S320-(D13S118, D13S153)-RB1-D13S319-D13S25-(D13S31, D13S59, D13S133, D13S137)-D13S163-D13S119-(D13S26, D13S55)-(D13S131, D13S134, D13S135, D13S144, D13S152)-TEL.

摘要

利用19个DNA标记和13个在该染色体区域存在断点的细胞系构建了染色体区域13q14 - q21的缺失杂交断点图谱。这些细胞系在该区域定义了10个不同的区间，该区域跨度约为20兆碱基对。这些标记包括6个限制性片段长度多态性（RFLP）标记、11个暂时定位到13q14 - 21区域的微卫星标记，以及2个从包含13pter - q14.3的细胞系ICD的EMBL3文库中开发的新的多态性CA重复序列。确定了以下标记顺序：着丝粒 - D13S320 -（D13S118，D13S153）- RB1 - D13S319 - D13S25 -（D13S31，D13S59，D13S133，D13S137）- D13S163 - D13S119 -（D13S26，D13S55）-（D13S131，D13S134，D13S135，D13S144，D13S152）- 端粒。