Donoso M V, Bates F, Montiel J, Huidobro-Toro J P
Departamento de Fisiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago.
Neurosci Lett. 1994 Mar 14;169(1-2):59-62. doi: 10.1016/0304-3940(94)90356-5.
Suramin (1-100 microM) and alpha, beta-methylene adenosine 5'-triphosphate (AMPCPP, 39 microM), antagonized the motor activity induced by exogenous adenosine 5'-triphosphate (ATP) but not exogenous noradrenaline (NA) in the longitudinal musculature of prostatic (P) and epididymal (E) segments of the rat vas deferens. Likewise, application of these drugs reduced the fast component of the nerve-stimulated contraction in response to a single transmural electrical pulse in E and P. Suramin also blocked in a concentration-dependent fashion, the contractile responses to trains of 1.5, 5, 15 or 30 Hz transmural electrical pulses in P, while it did not affect those in E. AMPCPP obliterated responses to trains of 1.5, 5, and 15 Hz in P, while reducing these responses in E to a significantly lesser extent. Present results strongly support that ATP is the motor transmitter in P, while in E, ATP and NA are likely the co-transmitters responsible for the motor tone.
苏拉明（1 - 100微摩尔）和α，β - 亚甲基腺苷5'-三磷酸（AMPCPP，39微摩尔），可拮抗外源性腺苷5'-三磷酸（ATP）诱导的大鼠输精管前列腺段（P）和附睾段（E）纵行肌的运动活性，但不拮抗外源性去甲肾上腺素（NA）诱导的运动活性。同样，应用这些药物可降低E段和P段对单个跨壁电脉冲刺激的神经刺激收缩的快速成分。苏拉明还以浓度依赖性方式阻断P段对1.5、5、15或30赫兹跨壁电脉冲串的收缩反应，而对E段则无影响。AMPCPP消除了P段对1.5、5和15赫兹电脉冲串的反应，而在E段则将这些反应降低到明显较小的程度。目前的结果有力地支持，ATP是P段的运动递质，而在E段，ATP和NA可能是负责运动张力的共同递质。
