Venanzetti F, Cesaroni D, Mariottini P, Sbordoni V
Dipartimento di Biologia, Università di Roma Tor Vergata, Italy.
Mol Phylogenet Evol. 1993 Dec;2(4):275-80. doi: 10.1006/mpev.1993.1026.
Five species of Dolichopoda cave crickets have been studied by means of mtDNA RFLPs. The phylogenetic relationships among them were previously inferred from two different molecular measures: allozyme polymorphisms and DNA-DNA hybridization. mtDNA data generate a phylogeny exactly matching those obtained from the other two genetic markers. This is not always the case for other organisms studied so far. This result is discussed in respect of the performance of the three molecular approaches and the population biology of these cave dwelling insects. A tentative calibration of mtDNA rate for Dolichopoda produces an estimate of about 2%/lineage/Myr. The comparison between mtDNA and scnDNA divergence estimates suggests that in these crickets changes accumulate approximately 2-3 times faster in mitochondrial than single copy nuclear sequences.
已通过线粒体DNA限制性片段长度多态性（mtDNA RFLPs）对五种洞栖长角蟋进行了研究。此前曾根据两种不同的分子指标推断它们之间的系统发育关系：等位酶多态性和DNA-DNA杂交。线粒体DNA数据生成的系统发育树与从其他两种遗传标记获得的系统发育树完全匹配。对于迄今为止研究的其他生物而言，情况并非总是如此。结合这三种分子方法的表现以及这些穴居昆虫的种群生物学对这一结果进行了讨论。对洞栖长角蟋线粒体DNA速率的初步校准得出的估计值约为2%/谱系/百万年。线粒体DNA与单拷贝核DNA分歧估计值之间的比较表明，在这些蟋蟀中，线粒体序列中的变化积累速度比单拷贝核序列快约2至3倍。
