Van Straaten J F, Dokter W H, Stulp B K, Vellenga E
Department of Medicine, University of Groningen, The Netherlands.
Cytokine. 1994 May;6(3):229-34. doi: 10.1016/1043-4666(94)90017-5.
Interleukin 5 (IL-5) and Interleukin 3 (IL-3) mRNA levels in human peripheral blood T cells were compared by semi-quantitative polymerase chain reaction (PCR) analysis. Unstimulated T Cells did not express IL-5 and IL-3 mRNA. IL-5 and IL-3 mRNA expression were similarly induced by the lectin concanavalin A (Con A). The protein kinase C (PKC) activator phorbol myristate acetate (PMA) triggered both IL-3 and IL-5 mRNA expression, whereby IL-5 and IL-3 mRNA expression was observed after 9 and 3 h treatment, respectively. Stimulation with calcium ionophore A23187 induced IL-3 mRNA expression, whereas it failed to induce IL-5 mRNA. In contrast to IL-3 mRNA, the expression of IL-5 mRNA was dependent on de novo protein synthesis, since cycloheximide (CHX) blocked the Con A plus PMA induced IL-5 mRNA expression. In contrast, cyclosporin A (CsA) inhibited but failed to completely block the expression of IL-3 and IL-5 mRNA. mRNA studies in T cell subsets revealed that the expression of IL-5 mRNA was restricted to the CD4 positive T cell subset in response to Con A plus PMA stimulation. On the other hand, IL-3 mRNA expression was noticed in both the CD4 and the CD8 positive T cell subset. These data indicate that the selective expression of IL-5 by human T cells can either be explained by activation of a selective intracellular signalling pathway or by selective activation of a T cell subset. Alternatively, both processes could be involved.
通过半定量聚合酶链反应（PCR）分析比较了人外周血T细胞中白细胞介素5（IL-5）和白细胞介素3（IL-3）的mRNA水平。未受刺激的T细胞不表达IL-5和IL-3 mRNA。凝集素刀豆球蛋白A（Con A）同样能诱导IL-5和IL-3 mRNA表达。蛋白激酶C（PKC）激活剂佛波酯肉豆蔻酸乙酸酯（PMA）可触发IL-3和IL-5 mRNA表达，分别在处理9小时和3小时后观察到IL-5和IL-3 mRNA表达。用钙离子载体A23187刺激可诱导IL-3 mRNA表达，而不能诱导IL-5 mRNA表达。与IL-3 mRNA不同，IL-5 mRNA的表达依赖于从头合成蛋白质，因为环己酰亚胺（CHX）可阻断Con A加PMA诱导的IL-5 mRNA表达。相比之下，环孢素A（CsA）可抑制但不能完全阻断IL-3和IL-5 mRNA的表达。T细胞亚群的mRNA研究表明，在Con A加PMA刺激下，IL-5 mRNA的表达仅限于CD4阳性T细胞亚群。另一方面，在CD4和CD8阳性T细胞亚群中均观察到IL-3 mRNA表达。这些数据表明，人T细胞对IL-5的选择性表达，要么可以通过选择性细胞内信号通路的激活来解释，要么可以通过T细胞亚群的选择性激活来解释。或者，这两个过程可能都参与其中。
