Okuzawa K, Franzén B, Lindholm J, Linder S, Hirano T, Bergman T, Ebihara Y, Kato H, Auer G
Department of Surgery, Tokyo Medical College.
Electrophoresis. 1994 Mar-Apr;15(3-4):382-90. doi: 10.1002/elps.1150150157.
Fourteen human lung tumors of various histopathological types were subjected to two-dimensional gel electrophoresis (2-DE). Samples were prepared for 2-DE using a nonenzymatic sample preparation (NESP) technique recently established in our laboratory. Variations in the expression of some polypeptides were observed between tumors of different histopathological types. To this end, high expression of beta-tubulin, heat shock proteins 73 and 90, lamin B, and proliferating cell nuclear antigen (PCNA) were observed in small cell lung carcinomas (SCLC). One polypeptide of unknown identity (35 kDa, pI 5.5) was significantly overexpressed in primary lung adenocarcinomas compared with SCLC, squamous cell lung carcinomas, metastatic lung adenocarcinomas from colon and rectum, and normal tissue. The amino acid composition of this polypeptide is presented. In summary, combining the NESP technique and 2-DE is an effective approach to define tumor-specific markers.
对14例不同组织病理学类型的人肺肿瘤进行了二维凝胶电泳（2-DE）。使用我们实验室最近建立的非酶样品制备（NESP）技术制备2-DE样品。在不同组织病理学类型的肿瘤之间观察到一些多肽表达的差异。为此，在小细胞肺癌（SCLC）中观察到β-微管蛋白、热休克蛋白73和90、核纤层蛋白B以及增殖细胞核抗原（PCNA）的高表达。与SCLC、肺鳞状细胞癌、来自结肠和直肠的转移性肺腺癌以及正常组织相比，一种身份不明的多肽（35 kDa，pI 5.5）在原发性肺腺癌中显著过表达。给出了该多肽的氨基酸组成。总之，将NESP技术和2-DE相结合是定义肿瘤特异性标志物的有效方法。
