Zanzinger J, Hoffmann I, Becker K
Institute for Animal Production in the Tropics and Subtropics, University of Hohenheim, Stuttgart, Germany.
Comp Biochem Physiol Comp Physiol. 1994 Jun-Jul;108(2-3):169-73. doi: 10.1016/0300-9629(94)90082-5.
In previous articles it has been shown that blood parameters may be useful to assess physical fitness in draught cattle. The aim of the present study was to detect possible variations in baseline values for the key metabolites: lactate and free fatty acids (FFA), and for blood gases in samples drawn from a catheterized jugular vein. Sampling took place immediately after venipuncture at intervals of 3 min for 1 hr in Simmental oxen (N = 6) and during a period of 24 hr at intervals of 60 min for Zebu (N = 4) and Simmental (N = 6) oxen. After puncture of the vein, plasma FFA and oxygen (pvO2) were elevated for approximately 15 min. All parameters returned to baseline values within 1 hr of the catheter being inserted. Twenty-four-hour mean baseline values for all measured parameters were significantly different (P < or = 0.001) between Zebu and Simmental. All parameters elicited diurnal variations which were mainly related to feed intake. The magnitude of these variations is comparable to the responses to light draught work. It is concluded that a strict standardization of blood sampling, at least in respect of time after feeding, is required for a reliable interpretation of endurance-indicating blood parameters measured under field conditions.
在之前的文章中已经表明，血液参数可能有助于评估役用牛的体能状况。本研究的目的是检测关键代谢物（乳酸和游离脂肪酸[FFA]）以及从颈静脉插管采集的样本中的血气的基线值可能存在的变化。在西门塔尔牛（N = 6）中，静脉穿刺后立即每隔3分钟采集一次样本，持续1小时；在瘤牛（N = 4）和西门塔尔牛（N = 6）中，在24小时内每隔60分钟采集一次样本。静脉穿刺后，血浆FFA和氧气（pvO2）升高约15分钟。插入导管后1小时内，所有参数均恢复到基线值。瘤牛和西门塔尔牛之间所有测量参数的24小时平均基线值存在显著差异（P≤0.001）。所有参数均呈现出日变化，主要与采食量有关。这些变化的幅度与轻度役用工作的反应相当。得出的结论是，为了可靠地解释在野外条件下测量的耐力指示性血液参数，至少在采食后的时间方面，需要对血液采样进行严格的标准化。
