Castel M, Hochman J
Cell Tissue Res. 1976 Oct 22;174(1):69-81. doi: 10.1007/BF00222151.
Vasopressin was immunohistochemically localized at the electron microscopic (EM) level in the hypothalamic-neurohypophysial system (HNS) of three murids. Antiserum to vasopressin was produced in rabbits injected with lysine vasopressin (LVP) conjugated to egg albumin (EA), anti-EA being precipitated prior to staining. Sternberger's unlabeled antibody peroxidase technique was employed, immunoreactivity being designated by peroxidase-anti-peroxidase (PAP) molecules and electron opacity. Immunoreactive neurosecretory granules (NSG) were found in the perikarya of the supraoptic nucleus (SON) in all three murids investigated, although far more profusely in the two wild strains. Immunoreactive axonal NSG were observed in the inner and outer zones of the median eminence (ME), and within most of the axons and terminals in the neurohypophysis. The concentration of primary serum effective for staining the SON (1:10-1:50) was far higher than that required for the ME and the neurohypophysis (1:500-1:1,200). Anti-LVP also induced electron opacity of granules in cells of the pars intermedia (PI). Discussion centers of the significance of immunoreactive NSG in the neurosecretory (NS) perikarya, on the possibility of an extragranular pool of hormone, and on speculation about the electron opacity of the PI granules.
采用免疫组织化学方法在电子显微镜（EM）水平对三只啮齿动物下丘脑-神经垂体系统（HNS）中的加压素进行定位。用与卵清蛋白（EA）偶联的赖氨酸加压素（LVP）对家兔进行注射，制备抗加压素抗血清，在染色前先沉淀抗EA。采用斯特恩伯格未标记抗体过氧化物酶技术，通过过氧化物酶-抗过氧化物酶（PAP）分子和电子不透明度来显示免疫反应性。在所研究的三只啮齿动物的视上核（SON）的核周体中均发现了免疫反应性神经分泌颗粒（NSG），不过在两个野生品系中更为丰富。在正中隆起（ME）的内区和外区以及神经垂体的大多数轴突和终末内均观察到了免疫反应性轴突NSG。对视上核进行染色有效的原血清浓度（1:10 - 1:50）远高于对正中隆起和神经垂体染色所需的浓度（1:500 - 1:1200）。抗LVP还诱导了中间部（PI）细胞中颗粒的电子不透明度。讨论了神经分泌（NS）核周体中免疫反应性NSG的意义、激素颗粒外池的可能性以及对中间部颗粒电子不透明度的推测。
