一种用于检测人血清中IgE的简单免疫酶法。
A simple immunoenzymatic method for measuring IgE in human sera.
作者信息
Gluesdon J L, Thierry R, Avrameas S
出版信息
Clin Exp Immunol. 1976 Jul;25(1):180-3.
Abstract
A reliable and relatively simple method for the estimation of serum IgE by single radial immunodiffusion is described. The method requires glucose oxidase-labelled antibodies. The method permits the measurement of IgE concentrations ranging from 20 to 700 i.u./ml. The values obtained in unknown samples were in good agreement with those obtained by radioimmunoassay (correlation coefficient r=0-9557).
摘要
本文描述了一种通过单向放射免疫扩散法估算血清IgE的可靠且相对简单的方法。该方法需要葡萄糖氧化酶标记的抗体。此方法可测量浓度范围为20至700国际单位/毫升的IgE。未知样品中获得的值与通过放射免疫测定法获得的值高度一致（相关系数r = 0 - 9557）。
相似文献
1
A simple immunoenzymatic method for measuring IgE in human sera.一种用于检测人血清中IgE的简单免疫酶法。
Clin Exp Immunol. 1976 Jul;25(1):180-3.
2
Quantitative estimation of IgE and IgD by laser nephelometry.
Allergol Immunopathol (Madr). 1979 Jul-Aug;7(4):289-96.
3
Evaluation of IgE serum level by radial immunodiffusion and radioimmunoassay in allergic diseases.采用放射免疫扩散法和放射免疫分析法评估过敏性疾病患者血清IgE水平。
Allergol Immunopathol (Madr). 1975 Jul-Aug;3(4):221-30.
4
[Quantification of total IgE on microtiter plates by enzyme immunoassay].[通过酶免疫测定法定量微孔板上的总免疫球蛋白E]
Allergol Immunopathol (Madr). 1982 Nov-Dec;10(6):423-8.
5
6
[A new 1-step immunoenzyme sandwich technic. Application to the determination of total serum IgE].[一种新的一步免疫酶夹心技术。在血清总IgE测定中的应用]
Pathol Biol (Paris). 1984 Jun;32(6):736-8.
7
[Immunoenzymatic method for the quantitative determination of specific IgE antibodies].
Recenti Prog Med. 1989 Jul-Aug;80(7-8):421-3.
8
[Usefulness of AlaSTAT, a new method for the measurement of IgE].
Arerugi. 1991 Apr;40(4):444-53.
9
A microtiter solid phase radioimmunoassay for total serum IgE.用于检测血清总IgE的微量滴定板固相放射免疫测定法。
Ann Allergy. 1981 Mar;46(3):132-6.
10
[Immunoenzymatic evaluation of serum IgE in onchocerciasis in Chiapas, Mexico].[墨西哥恰帕斯州盘尾丝虫病患者血清IgE的免疫酶法评估]
Salud Publica Mex. 1989 Nov-Dec;31(6):772-8.
引用本文的文献
1
Enzyme-linked immunosorbent assay for measuring serum IgE.用于测量血清免疫球蛋白E的酶联免疫吸附测定法。
Experientia. 1980 Sep 15;36(9):1122. doi: 10.1007/BF01966006.
本文引用的文献
1
QUANTITATIVE DETERMINATION OF SERUM IMMUNOGLOBULINS IN ANTIBODY-AGAR PLATES.抗体琼脂平板中血清免疫球蛋白的定量测定
J Immunol. 1965 Jan;94:84-90.
2
Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies.用戊二醛将酶与蛋白质偶联。偶联物用于抗原和抗体的检测。
Immunochemistry. 1969 Jan;6(1):43-52. doi: 10.1016/0019-2791(69)90177-3.
3
Immunochemical quantitation of antigens by single radial immunodiffusion.通过单向辐射免疫扩散法对抗原进行免疫化学定量。
Immunochemistry. 1965 Sep;2(3):235-54. doi: 10.1016/0019-2791(65)90004-2.
4
Studies on single radial immunodiffusion techniques for the quantitation of antigen and antibody.用于抗原和抗体定量的单向放射免疫扩散技术研究。
J Immunol Methods. 1972 Jan;1(2):123-31. doi: 10.1016/0022-1759(72)90039-7.
5
Measurement of serum IgE by a one-step single radial radiodiffusion method.
J Allergy Clin Immunol. 1972 Feb;49(2):72-80. doi: 10.1016/0091-6749(72)90058-9.
6
Properties of myeloma immunoglobulin E(Yu). Chemical, fluorescence polarisation and spin-labeled studies.骨髓瘤免疫球蛋白E（宇）的特性。化学、荧光偏振和自旋标记研究。
Immunochemistry. 1973 Oct;10(10):681-8. doi: 10.1016/0019-2791(73)90211-5.
7
Radioactive single radial diffusion: a method for increasing the sensitivity of immunochemical quantification of proteins in agar gel.放射性单向扩散：一种提高琼脂凝胶中蛋白质免疫化学定量灵敏度的方法。
Bull World Health Organ. 1969 Apr;40(4):613-6.
8
The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents.蛋白质与戊二醛的交联及其在免疫吸附剂制备中的应用。
Immunochemistry. 1969 Jan;6(1):53-66. doi: 10.1016/0019-2791(69)90178-5.
9
An immunoenzymatic method for measuring low concentrations of antigens by single radial diffusion.
Immunochemistry. 1974 Sep;11(9):595-8. doi: 10.1016/0019-2791(74)90251-1.
10
Polyacrylamide-protein immunoadsorbents prepared with glutaraldehyde.用戊二醛制备的聚丙烯酰胺-蛋白质免疫吸附剂。
FEBS Lett. 1972 Jun 1;23(1):24-8. doi: 10.1016/0014-5793(72)80274-6.
Zotero 插件