Otsuka H, Lindahl S G, Lagercrantz H, Yamamoto Y
Department of Anesthesiology and Intensive Care, Karolinska Hospital, Stockholm, Sweden.
Neurosci Lett. 1994 Apr 25;171(1-2):94-6. doi: 10.1016/0304-3940(94)90613-0.
We examined the effects of N-methyl-D-aspartate (NMDA) antagonists, 2-amino-5-phosphonovaleric acid (APV) and non-NMDA antagonists, 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) on inspiratory neurons in the medulla oblongata and C4 and C5 ventral root activity in the in vitro brainstem-spinal cord preparation of newborn rats. Application of APV induced increased rate of inspiratory neuron bursts, and decreased burst duration. The C4 burst activity was almost abolished by application of CNQX, whereas inspiratory neurons continued to discharge rhythmically. The results suggest that non-NMDA receptors are not crucially involved in the medullary respiratory rhythmogenesis, and that NMDA-mediated neurotransmission could modify the respiratory pattern, in the in vitro model.
我们研究了 N-甲基-D-天冬氨酸（NMDA）拮抗剂 2-氨基-5-磷酸戊酸（APV）和非 NMDA 拮抗剂 6-氰基-7-硝基喹喔啉-2,3-二酮（CNQX）对新生大鼠离体脑干-脊髓标本延髓吸气神经元以及 C4 和 C5 腹根活动的影响。应用 APV 可使吸气神经元爆发频率增加，爆发持续时间缩短。应用 CNQX 几乎可消除 C4 爆发活动，而吸气神经元仍有节律地放电。结果表明，在该离体模型中，非 NMDA 受体并非延髓呼吸节律产生的关键因素，而 NMDA 介导的神经传递可改变呼吸模式。
