Takahashi H, Shintani Y, Okauchi T, Ishikawa M, Bando H, Azekawa T, Morita Y, Saito S
First Department of Internal Medicine, School of Medicine, University of Tokushima, Japan.
J Neurosci Methods. 1994 Apr;52(1):33-8. doi: 10.1016/0165-0270(94)90052-3.
We determined the most suitable conditions for measuring the somatostatin (SRIF) level by brain microdialysis and investigated its release from the hypothalamus. The relative recovery rate of SRIF was 8.4 +/- 0.5% (mean +/- SE) using a polycarbonate (PC) membrane with the push-pull method at a flow rate of 2 microliters/min. Using tubes with an internal diameter of 0.28 mm and lengths of 5, 25, 50 and 100 cm, the relative recovery rates using a PC membrane with the push method were 8.2 +/- 0.5%, 7.3 +/- 0.6%, 6.2 +/- 0.5% and 4.1 +/- 0.6%, respectively. When using tubes with an internal diameter of 0.1 mm and lengths of 5, 25, 50 and 100 cm, the relative recovery rates were 7.3 +/- 0.7%, 5.6 +/- 1.0%, 3.5 +/- 1.1% and 1.4 +/- 0.7%, respectively. The relative recovery rate was 5.2 +/- 0.5% with a polysulfone (PS-F, Fresenius) membrane, 4.5 +/- 0.4% with a PS-H (Hospal) membrane, 2.6 +/- 0.2% with an ethylenevinyl alcohol membrane (EVAL), 5.1 +/- 0.8% with a polyvinyl alcohol (PVA) membrane and 10.4 +/- 0.8% with a PS-K (Kaneka) membrane. With the push method, the extracellular SRIF level in rat pituitary was 42.8 +/- 1.8 pg/ml with a PC membrane, 23.1 +/- 2.9 pg/ml with an EVAL membrane at a flow rate of 2 microliters/min. With the push-pull method, it was 52.7 +/- 5.2 pg/ml using a PC membrane, 33.5 +/- 2.8 pg/ml using a PVA membrane and 54.4 +/- 3.2 pg/ml using a PS-K membrane.(ABSTRACT TRUNCATED AT 250 WORDS)
我们确定了通过脑微透析测量生长抑素（SRIF）水平的最合适条件，并研究了其从下丘脑的释放情况。使用聚碳酸酯（PC）膜，采用推挽法，流速为2微升/分钟时，SRIF的相对回收率为8.4±0.5%（平均值±标准误）。使用内径为0.28毫米、长度分别为5、25、50和100厘米的管子，采用推法时，使用PC膜的相对回收率分别为8.2±0.5%、7.3±0.6%、6.2±0.5%和4.1±0.6%。当使用内径为0.1毫米、长度分别为5、25、50和100厘米的管子时，相对回收率分别为7.3±0.7%、5.6±1.0%、3.5±1.1%和1.4±0.7%。聚砜（PS - F，费森尤斯）膜的相对回收率为5.2±0.5%，PS - H（霍斯帕尔）膜为4.5±0.4%，乙烯 - 乙烯醇膜（EVAL）为2.6±0.2%，聚乙烯醇（PVA）膜为5.1±0.8%，PS - K（钟渊化学）膜为10.4±0.8%。采用推法时，在流速为2微升/分钟的情况下，大鼠垂体中细胞外SRIF水平，使用PC膜时为42.8±1.8皮克/毫升，使用EVAL膜时为23.1±2.9皮克/毫升。采用推挽法时，使用PC膜为52.7±5.2皮克/毫升，使用PVA膜为33.5±2.8皮克/毫升，使用PS - K膜为54.4±3.2皮克/毫升。（摘要截于250字）
