胆碱能刺激通过对胸腺上皮的烟碱样作用调节小鼠胸腺细胞的凋亡和分化。

Cholinergic stimulation modulates apoptosis and differentiation of murine thymocytes via a nicotinic effect on thymic epithelium.

作者信息

Rinner I, Kukulansky T, Felsner P, Skreiner E, Globerson A, Kasai M, Hirokawa K, Korsatko W, Schauenstein K

机构信息

Institute of General and Experimental Pathology, University of Graz, Austria.

出版信息

Biochem Biophys Res Commun. 1994 Sep 15;203(2):1057-62. doi: 10.1006/bbrc.1994.2289.

DOI:10.1006/bbrc.1994.2289

PMID:7916567

Abstract

Effects of cholinergic agonists/antagonists on apoptosis and differentiation of murine thymus cells were investigated in two in vitro models. Treatment with 10(-7) M carbachol was found to counteract the effects of a cortical thymus epithelial cell line (TEC 1.4), on apoptosis and the ratio of CD4 CD8 DP/DN cells in cocultured fetal thymus lobes. A medullary line (TEC 2.3) did not influence apoptosis in fetal thymus lobes. Both TEC lines had the same strong apoptotic effect on thymus cells in suspension, but only the effect of TEC 1.4 was counteracted by carbachol. This cholinergic influence on TEC 1.4 cells is mediated via nicotinic cholinergic receptors, since d-tubocurarine, but not atropine, effectively blocked the effect of carbachol. The results suggest that cholinergic signals to thymic epithelial cells may have regulatory influence on thymic differentiation and selection processes.

摘要

在两个体外模型中研究了胆碱能激动剂/拮抗剂对小鼠胸腺细胞凋亡和分化的影响。发现用10(-7)M卡巴胆碱处理可抵消皮质胸腺上皮细胞系（TEC 1.4）对共培养的胎儿胸腺叶中细胞凋亡及CD4 CD8双阳性/双阴性细胞比例的影响。一个髓质细胞系（TEC 2.3）不影响胎儿胸腺叶中的细胞凋亡。两个TEC细胞系对悬浮的胸腺细胞都有相同的强烈凋亡作用，但只有TEC 1.4的作用被卡巴胆碱抵消。这种对TEC 1.4细胞的胆碱能影响是通过烟碱型胆碱能受体介导的，因为d-筒箭毒碱而非阿托品能有效阻断卡巴胆碱的作用。结果表明，向胸腺上皮细胞传递的胆碱能信号可能对胸腺分化和选择过程具有调节作用。