Kemp S J, Maillard J C, Teale A J
International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
Anim Genet. 1993 Apr;24(2):125-7. doi: 10.1111/j.1365-2052.1993.tb00253.x.
A polymorphism was detected in the 3' untranslated region of the bovine gamma-S-crystallin gene by direct sequencing of polymerase chain reaction (PCR) products from genomic DNA of an N'Dama bull and a Boran cow. A set of three PCR primers was designed to detect this difference and thus give allele-specific amplification. The two allele-specific primers differ in length by 20 nucleotides so that the allelic products may be distinguished by simple agarose gel electrophoresis following a single PCR reaction. This provides a simple and rapid assay for this polymorphism.
