Yu X, Chung M, Morabito M A, Barnstable C J
Interdepartmental Neuroscience Program, Yale University School of Medicine, New Haven, CT 06510.
Biochem Biophys Res Commun. 1993 Feb 26;191(1):76-82. doi: 10.1006/bbrc.1993.1186.
DNase I protection and gel retardation assays have identified two sequences 5' to the rat opsin gene that interact with nuclear proteins from retina but not from a number of neuronal and non-neuronal tissues. These sites, Ret 2 and Ret 3, are over 1200 base pairs apart but seem to interact with the same protein(s). Synthetic oligonucleotides corresponding to each site were able to inhibit complex formation in a gel retardation assay using an oligonucleotide corresponding to the other site. The proteins binding to the Ret 2 and Ret 3 sites co-eluted in both ion exchange and gel filtration chromatography. The protein(s) were also present at adult levels at birth, suggesting that they may represent differentiation products expressed in the proliferating retinal epithelium.
脱氧核糖核酸酶I保护试验和凝胶阻滞试验已鉴定出大鼠视蛋白基因5'端的两个序列，它们与来自视网膜的核蛋白相互作用，但不与许多神经组织和非神经组织的核蛋白相互作用。这些位点，即Ret 2和Ret 3，相距超过1200个碱基对，但似乎与相同的蛋白质相互作用。在凝胶阻滞试验中，对应于每个位点的合成寡核苷酸能够使用对应于另一个位点的寡核苷酸抑制复合物的形成。与Ret 2和Ret 3位点结合的蛋白质在离子交换色谱和凝胶过滤色谱中共同洗脱。这些蛋白质在出生时也处于成年水平，这表明它们可能代表在增殖性视网膜上皮中表达的分化产物。
