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1
Common sequence motifs coding for higher-plant and prokaryotic O-acetylserine (thiol)-lyases: bacterial origin of a chloroplast transit peptide?编码高等植物和原核生物O-乙酰丝氨酸（硫醇）裂解酶的常见序列基序：叶绿体转运肽的细菌起源？
Biochem J. 1993 Aug 1;293 ( Pt 3)(Pt 3):829-33. doi: 10.1042/bj2930829.
2
O-acetylserine(thiol)lyase from spinach (Spinacia oleracea L.) leaf: cDNA cloning, characterization, and overexpression in Escherichia coli of the chloroplast isoform.菠菜（Spinacia oleracea L.）叶片中的O-乙酰丝氨酸（硫醇）裂解酶：叶绿体同工型的cDNA克隆、特性分析及在大肠杆菌中的过表达
Arch Biochem Biophys. 1993 Jan;300(1):213-22. doi: 10.1006/abbi.1993.1030.
3
Spinach chloroplast 0-acetylserine (thiol)-lyase exhibits two catalytically non-equivalent pyridoxal-5'-phosphate-containing active sites.菠菜叶绿体O-乙酰丝氨酸（硫醇）-裂解酶具有两个催化不等价的含磷酸吡哆醛-5'-磷酸的活性位点。
Eur J Biochem. 1996 Feb 15;236(1):272-82. doi: 10.1111/j.1432-1033.1996.00272.x.
4
Isolation and characterization of cDNA that encodes a putative mitochondrion-localizing isoform of cysteine synthase (O-acetylserine(thiol)-lyase) from Spinacia oleracea.菠菜中编码假定定位于线粒体的半胱氨酸合酶（O-乙酰丝氨酸（硫醇）裂解酶）同工型的cDNA的分离与鉴定。
J Biol Chem. 1994 Nov 11;269(45):28187-92.
5
Tobacco plants transformed with the O-acetylserine (thiol) lyase gene of wheat are resistant to toxic levels of hydrogen sulphide gas.用小麦的O-乙酰丝氨酸（硫醇）裂解酶基因转化的烟草植株对硫化氢气体的毒性水平具有抗性。
Plant J. 1993 Nov;4(5):759-69. doi: 10.1046/j.1365-313x.1993.04050759.x.
6
Purification and characterization of O-acetylserine (thiol) lyase from spinach chloroplasts.菠菜叶绿体中O-乙酰丝氨酸（硫醇）裂解酶的纯化与特性分析
Arch Biochem Biophys. 1992 Jun;295(2):379-90. doi: 10.1016/0003-9861(92)90531-z.
7
Modulation of cysteine biosynthesis in chloroplasts of transgenic tobacco overexpressing cysteine synthase [O-acetylserine(thiol)-lyase].过表达半胱氨酸合酶[O-乙酰丝氨酸（硫醇）裂解酶]的转基因烟草叶绿体中半胱氨酸生物合成的调控
Plant Physiol. 1994 Nov;106(3):887-95. doi: 10.1104/pp.106.3.887.
8
Cysteine biosynthesis in Chlamydomonas reinhardtii. Molecular cloning and regulation of O-acetylserine(thiol)lyase.莱茵衣藻中的半胱氨酸生物合成。O-乙酰丝氨酸（硫醇）裂解酶的分子克隆与调控。
Eur J Biochem. 1999 Sep;264(3):848-53. doi: 10.1046/j.1432-1327.1999.00676.x.
9
Plant aldolase: cDNA and deduced amino-acid sequences of the chloroplast and cytosol enzyme from spinach.植物醛缩酶：菠菜叶绿体和细胞质酶的cDNA及推导的氨基酸序列
Plant Mol Biol. 1993 Jan;21(2):331-40. doi: 10.1007/BF00019948.
10
Phylogeny of metabolic pathways: O-acetylserine sulphydrylase A is homologous to the tryptophan synthase beta subunit.代谢途径的系统发育：O-乙酰丝氨酸巯基酶A与色氨酸合成酶β亚基同源。
Mol Microbiol. 1988 Nov;2(6):777-83. doi: 10.1111/j.1365-2958.1988.tb00089.x.

引用本文的文献

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RASPBERRY3 gene encodes a novel protein important for embryo development.RASPBERRY3基因编码一种对胚胎发育很重要的新型蛋白质。
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本文引用的文献

1
Subcellular Distribution of O-Acetylserine(thiol)lyase in Cauliflower (Brassica oleracea L.) Inflorescence.花椰菜（甘蓝型油菜）花中 O-乙酰丝氨酸（硫醇）裂解酶的亚细胞分布。
Plant Physiol. 1992 Mar;98(3):927-35. doi: 10.1104/pp.98.3.927.
2
Localization of ATP Sulfurylase and O-Acetylserine(thiol)lyase in Spinach Leaves.在菠菜叶片中定位三磷酸腺苷硫酸化酶和 O-乙酰丝氨酸（巯基）裂解酶。
Plant Physiol. 1990 Nov;94(3):1345-52. doi: 10.1104/pp.94.3.1345.
3
On the origin of mitosing cells.关于有丝分裂细胞的起源
J Theor Biol. 1967 Mar;14(3):255-74. doi: 10.1016/0022-5193(67)90079-3.
4
O-acetylserine(thiol)lyase from spinach (Spinacia oleracea L.) leaf: cDNA cloning, characterization, and overexpression in Escherichia coli of the chloroplast isoform.菠菜（Spinacia oleracea L.）叶片中的O-乙酰丝氨酸（硫醇）裂解酶：叶绿体同工型的cDNA克隆、特性分析及在大肠杆菌中的过表达
Arch Biochem Biophys. 1993 Jan;300(1):213-22. doi: 10.1006/abbi.1993.1030.
5
Birth of a unique enzyme from an alternative reading frame of the preexisted, internally repetitious coding sequence.一种独特的酶由预先存在的内部重复编码序列的替代阅读框产生。
Proc Natl Acad Sci U S A. 1984 Apr;81(8):2421-5. doi: 10.1073/pnas.81.8.2421.
6
Enzymatic proof for the identity of the S-sulfocysteine synthase and cysteine synthase B of Salmonella typhimurium.鼠伤寒沙门氏菌S-磺基半胱氨酸合酶与半胱氨酸合酶B同一性的酶学证据。
J Bacteriol. 1984 Jun;158(3):1122-7. doi: 10.1128/jb.158.3.1122-1127.1984.
7
Evidence that thiosulfate assimilation by Salmonella typhimurium is catalyzed by cysteine synthase B.鼠伤寒沙门氏菌对硫代硫酸盐的同化作用由半胱氨酸合酶B催化的证据。
J Bacteriol. 1983 Nov;156(2):656-62. doi: 10.1128/jb.156.2.656-662.1983.
8
Pleiotrophy in a cysteine-requiring mutant of Samonella typhimurium resulting from altered protein-protein interaction.鼠伤寒沙门氏菌半胱氨酸需求突变体中的多效性源于蛋白质-蛋白质相互作用的改变。
J Biol Chem. 1969 Nov 10;244(21):6023-30.
9
The structural gene for O-acetylserine sulfhydrylase A in Salmonella typhimurium. Identity with the trzA locus.鼠伤寒沙门氏菌中O-乙酰丝氨酸巯基酶A的结构基因。与trzA位点的一致性。
J Biol Chem. 1974 Feb 10;249(3):867-72.
10
Evidence in favor of the symbiotic origin of chloroplasts: primary structure and evolution of tobacco glyceraldehyde-3-phosphate dehydrogenases.支持叶绿体共生起源的证据：烟草3-磷酸甘油醛脱氢酶的一级结构与进化
Cell. 1986 Oct 10;47(1):73-80. doi: 10.1016/0092-8674(86)90367-3.

编码高等植物和原核生物O-乙酰丝氨酸（硫醇）裂解酶的常见序列基序：叶绿体转运肽的细菌起源？

Common sequence motifs coding for higher-plant and prokaryotic O-acetylserine (thiol)-lyases: bacterial origin of a chloroplast transit peptide?

作者信息

Rolland N, Job D, Douce R

机构信息

Unité Mixte C.N.R.S./Rhône-Poulenc Agrochimie (U.M. 41), Centre National de la Recherche Scientifique, Lyon, France.

出版信息

Biochem J. 1993 Aug 1;293 ( Pt 3)(Pt 3):829-33. doi: 10.1042/bj2930829.

DOI:10.1042/bj2930829
PMID:7916619
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1134443/
Abstract

A comparison of the amino acid sequence of O-acetylserine (thiol)-lyase (EC 4.2.99.8) from Escherichia coli and the isoforms of this enzyme found in the cytosolic and chloroplastic compartments of spinach (Spinacia oleracea) leaf cells allows the essential lysine residue involved in the binding of the pyridoxal 5'-phosphate cofactor to be identified. The results of further sequence comparison of cDNAs coding for these proteins are discussed in the frame of the endosymbiotic theory of chloroplast evolution. The results are compatible with a mechanism in which the chloroplast enzyme originated from the cytosolic enzyme and both plant genes originated from a common prokaryotic ancestor. The comparison also suggests that the 5'-non-coding sequence of the bacterial gene was transferred to the plant cell nucleus and that it has been used to create the N-terminal portions of both plant enzymes, and possibly the transit peptide of the chloroplast enzyme.

摘要

对来自大肠杆菌的O-乙酰丝氨酸（硫醇）裂合酶（EC 4.2.99.8）的氨基酸序列与在菠菜（Spinacia oleracea）叶细胞的胞质溶胶和叶绿体区室中发现的该酶的同工型进行比较，可以鉴定出参与磷酸吡哆醛辅因子结合的必需赖氨酸残基。在叶绿体进化的内共生理论框架内讨论了编码这些蛋白质的cDNA的进一步序列比较结果。结果与一种机制相符，即叶绿体酶起源于胞质溶胶酶，并且两个植物基因都起源于一个共同的原核祖先。该比较还表明，细菌基因的5'-非编码序列转移到了植物细胞核中，并且已被用于创建两种植物酶的N端部分，可能还有叶绿体酶的转运肽。