小鼠核纤层蛋白A cDNA的核苷酸序列及其推导的氨基酸序列。

Nucleotide sequence of a mouse lamin A cDNA and its deduced amino acid sequence.

作者信息

Nakajima N, Sado T

机构信息

Department of Chemistry II, Faculty of Science, Hokkaido University, Sapporo, Japan.

出版信息

Biochim Biophys Acta. 1993 Jan 23;1171(3):311-4. doi: 10.1016/0167-4781(93)90072-l.

DOI:10.1016/0167-4781(93)90072-l

PMID:7916626

Abstract

We have cloned and determined the nucleotide sequence of a mouse lamin A cDNA. The clone contained the C-terminal two-thirds of the lamin A coding sequence and a 3' untranslated sequence with a poly(A) stretch. As has been reported for human lamin A/C cDNAs, a large part of the 5' sequence of our mouse lamin A clone was essentially identical with a previously reported mouse lamin C cDNA sequence, and the deduced C-terminal amino acid sequence shared strong homology with the human lamin A sequence. A putative deduced amino acid sequence for mouse lamin A, which was derived from our sequence and the published lamin C sequence, was 665 amino acids long. The degree of overall homology to the human sequence was more than 95%, and relatively more variation was scattered in the C-terminal lamin A-specific region.

摘要

我们克隆并测定了小鼠核纤层蛋白A cDNA的核苷酸序列。该克隆包含核纤层蛋白A编码序列的C端三分之二以及带有poly(A)序列的3'非翻译序列。正如已报道的人类核纤层蛋白A/C cDNA一样，我们的小鼠核纤层蛋白A克隆的5'序列大部分与先前报道的小鼠核纤层蛋白C cDNA序列基本相同，并且推导的C端氨基酸序列与人类核纤层蛋白A序列具有很强的同源性。从小鼠核纤层蛋白A的推测推导氨基酸序列（该序列来自我们测定的序列和已发表的核纤层蛋白C序列）长665个氨基酸。与人类序列的总体同源性程度超过95%，并且相对更多的变异分散在C端核纤层蛋白A特异性区域。