来自一名11周大人类胎儿大脑的编码铁蛋白H链的cDNA序列。

Sequence of a cDNA encoding the ferritin H-chain from an 11-week-old human fetal brain.

作者信息

Dhar M, Chauthaiwale V, Joshi J G

机构信息

Department of Biochemistry, University of Tennessee, Knoxville 37996-0840.

出版信息

Gene. 1993 Apr 30;126(2):275-8. doi: 10.1016/0378-1119(93)90380-l.

DOI:10.1016/0378-1119(93)90380-l

PMID:7916709

Abstract

A cDNA library in lambda Charon BS(-) from 11-week-old human fetal brain (FB) was screened using a human liver ferritin (FTH)-encoding cDNA as a probe. The complete sequence of the positive clone, cFB1, showed that the coding region and a part of the 5' and 3' untranslated regions (UTR) are identical to the corresponding published sequence of the liver cDNA. However, a particularly noteworthy difference is the presence of 279 bp of additional sequence in the FB 3'-UTR. Northern blot analysis of FB poly(A)+RNA showed it to be a part of the FTH transcript. Comparison of the 279-bp sequence with the GenBank and EMBL databases showed it to be 94.1, 62.5, and 58.9% similar to segments from human, mouse and rat FTH genomic sequences, respectively. However, in all these cases, only a part of this 279-bp sequence has been found in the nontranscribed region. We therefore conclude that in FB, the 279-bp sequence is a part of the mature FTH mRNA. Sequence analysis also suggests a differential poly(A) site selection in the production of FTH mRNA in FB and liver.

摘要

使用人肝铁蛋白（FTH）编码cDNA作为探针，对来自11周龄人胎儿脑（FB）的λ噬菌体Charon BS(-) cDNA文库进行筛选。阳性克隆cFB1的完整序列显示，其编码区以及5'和3'非翻译区（UTR）的一部分与已发表的肝脏cDNA相应序列相同。然而，一个特别值得注意的差异是FB的3'-UTR中存在279 bp的额外序列。对FB多聚腺苷酸加尾RNA（poly(A)+RNA）的Northern印迹分析表明，它是FTH转录本的一部分。将这279 bp的序列与GenBank和EMBL数据库进行比较，结果显示它分别与人、小鼠和大鼠FTH基因组序列的片段有94.1%、62.5%和58.9%的相似性。然而，在所有这些情况下，仅在非转录区域中发现了该279 bp序列的一部分。因此，我们得出结论，在FB中，279 bp序列是成熟FTH mRNA的一部分。序列分析还表明，在FB和肝脏中FTH mRNA产生过程中存在多聚腺苷酸化位点的差异选择。