Srikantha T, Soll D R
Department of Biology, University of Iowa, Iowa City 52242-1324.
Gene. 1993 Sep 6;131(1):53-60. doi: 10.1016/0378-1119(93)90668-s.
The Candida albicans strain WO-1 cells switch spontaneously, frequently, and reversibly between white and opaque colony-forming units which differ dramatically in their budding phenotypes. By screening a subtracted white cDNA library, the first white-specific cDNA, cWh11, was isolated and sequenced. This, in turn, was used to clone the Wh11 gene. Wh11 shows significant homology to the glucose/lipid-regulated GLP1 gene of Saccharomyces cerevisiae. Upon temperature-induced mass conversion from opaque to white colonies, transcription of Wh11 is abruptly activated at the second cell doubling, concomitant with commitment to the white phenotype. Wh11 exhibits the unique characteristics of being regulated not only by switching, but also by the bud-hypha transition.
白色念珠菌菌株WO-1细胞可在白色和不透明菌落形成单位之间自发、频繁且可逆地转换，它们的芽殖表型差异很大。通过筛选消减白色cDNA文库，分离并测序了首个白色特异性cDNA，即cWh11。进而利用该cDNA克隆了Wh11基因。Wh11与酿酒酵母的葡萄糖/脂质调节GLP1基因具有显著同源性。在温度诱导下从不透明菌落大量转变为白色菌落时，Wh11的转录在第二次细胞加倍时突然被激活，同时确定为白色表型。Wh11不仅受转换调节，还受芽-菌丝转变调节，具有独特的特征。
