Lorenzato M, Lallemand A, Visseaux-Coletto B, Gaillard D
Laboratoire Pol Bouin, CHRU Maison Blanche, Reims.
Ann Pathol. 1994;14(4):257-60.
With the new softwares that are available, it is now possible to measure the cellular DNA content on Feulgen stained paraffin sections, without enzymatic dissociation of the samples. The main advantage of this technique is to preserve the cellular histological context and to select the right cells that have to be analyzed. The application is of course to obtain information about cancer prognosis, but it can also be very useful in non tumoral pathology. The DNA measurement on paraffin sections can be applied to the diagnosis of triploidy or tetraploïdy in spontaneous abortions without any karyotype.
有了现有的新软件，现在可以在福尔根染色的石蜡切片上测量细胞DNA含量，而无需对样本进行酶解。这项技术的主要优点是保留细胞的组织学背景，并选择需要分析的合适细胞。其应用当然是获取有关癌症预后的信息，但在非肿瘤病理学中也可能非常有用。石蜡切片上的DNA测量可用于诊断自然流产中的三倍体或四倍体，而无需进行任何核型分析。
