Filkorn R, Wiedenmann A, Botzenhart K
Abteilung für Allgemeine Hygiene und Umwelthygiene, Eberhard-Karls-Universität Tübingen.
Zentralbl Hyg Umweltmed. 1994 Jun;195(5-6):489-94.
Although the diagnosis of cryptosporidiosis in diarrheic patients is relatively simple, as large amounts of oocysts are usually shed, environmental samples can contain only few oocysts which have a comparatively high epidemiological relevance. Very sensitive detection methods are therefore requested in environmental hygiene. Additionally these methods should allow a statement about the viability of the detected organisms. The combination of a DNA-digest and a viability assay followed by a PCR might reveal a very promising method. A DNA-digest destroys free DNA in the sample. In the following excystation protocol only viable sporozoites excyst. A PCR carried out now gives definitive proof of viable oocysts. Dead sporozoites within intact oocyst-walls are not detected.
