Zwillich S H, Fang Q, Kieber-Emmons T, Vonfeldt J, Monos D, Ramanujam T, Wang B, Weiner D B, Willams W V
Department of Medicine, Clinical Immunology/Rheumatology Unit, University of Rochester Medical Center, NY 14642.
DNA Cell Biol. 1994 Sep;13(9):923-31. doi: 10.1089/dna.1994.13.923.
While many investigators have examined V gene usage by the clonotypic T-cell receptor (TCR) in rheumatoid arthritis (RA) joints, few have reported on arthritic controls. We compared TCR alpha-chain V gene usage in knee synovial tissue specimens from 9 RA and 5 osteoarthritis (OA) patients. There was no significant difference in the number of V gene families used in RA compared with OA synovium. However, there was an increased prevalence of V alpha 28, V alpha 10, V alpha 17, and V alpha 18 and under representation of V alpha 15 in RA compared with OA synovium. Of these, V alpha 28 was also recently described by us as being present in RA synovial tissue early in the course of disease. V alpha 28 associated J region usage, and N-regional diversity was surveyed in T-cell receptors from additional rheumatoid synovial tissue T-cell populations and normal peripheral blood. Oligoclonality was observed in 6/10 rheumatoid specimens either by direct sequencing or where three or more molecular clones were sequenced, compared with 0/5 normal PBMCs. The oligoclonal populations included 2/3 cell lines stimulated with interleukin-2 (IL-2) alone. Several novel J regions were observed, with some recurrent residues observed at N-region positions. These data indicate an increased prevalence of certain TCR V region families in RA versus OA synovium, and suggest an antigen-driven expansion of V alpha 28-expressing T cells in RA synovium.
虽然许多研究人员已经研究了类风湿性关节炎（RA）关节中克隆型T细胞受体（TCR）的V基因使用情况，但很少有人报道关节炎对照的情况。我们比较了9例RA患者和5例骨关节炎（OA）患者膝关节滑膜组织标本中TCRα链V基因的使用情况。与OA滑膜相比，RA中使用的V基因家族数量没有显著差异。然而，与OA滑膜相比，RA中Vα28、Vα10、Vα17和Vα18的患病率增加，而Vα15的表达不足。其中，我们最近还描述了Vα28在疾病早期存在于RA滑膜组织中。在来自其他类风湿滑膜组织T细胞群体和正常外周血的T细胞受体中，对Vα28相关的J区域使用情况和N区域多样性进行了调查。通过直接测序或对三个或更多分子克隆进行测序，在10例类风湿标本中有6例观察到寡克隆性，而正常PBMCs为0/5。寡克隆群体包括仅用白细胞介素-2（IL-2）刺激的2/3细胞系。观察到几个新的J区域，在N区域位置观察到一些重复的残基。这些数据表明，与OA滑膜相比，RA中某些TCR V区域家族的患病率增加，并提示RA滑膜中表达Vα28的T细胞存在抗原驱动的扩增。
