A general method for immobilization of glycoproteins on regenerable immobilized metal-ion carriers: application to glucose oxidase from Penicillium chrysogenum and horseradish peroxidase.

A general method for immobilization of glycoproteins on immobilized metal-ion carriers is described. The method includes oxidation of the carbohydrate moiety of the glycoprotein with potassium periodate, covalent modification with histidine and immobilization on bivalent metal ion-iminodiacetic acid-agarose. The method is exemplified with horseradish peroxidase and glucose oxidase from Penicillium chrysogenum. The modified and immobilized enzymes are stable for at least 2 months at 4 degrees C. The immobilized enzymes were used as enzyme electrodes for the determination of the glucose concentration.