Paoletti P, Ascher P
Laboratoire de Neurobiologie, Ecole Normale Supérieure, Paris, France.
Neuron. 1994 Sep;13(3):645-55. doi: 10.1016/0896-6273(94)90032-9.
Changes in osmotic and hydrostatic pressure were found to modulate NMDA responses of cultured embryonic mouse neurons recorded in various patch-clamp configurations. In nucleated patches, NMDA currents were potentiated by reductions in external osmolarity and were reduced in hyper-osmotic solutions. These changes, which were greater for low concentrations of NMDA, were not observed for responses to kainate, glycine, or GABA. They could be mimicked by directly changing the pipette pressure in nucleated, outside-out, inside-out, and cell-attached patches. Osmosensitivity of NMDA responses was also observed in the whole-cell mode, but only after prolonged dialysis. Mechanosensitivity of NMDA receptors could have an important role in neuronal regions experiencing changes in membrane tension, such as spines or growth cones.
研究发现，渗透压和流体静压的变化可调节在各种膜片钳配置下记录的培养胚胎小鼠神经元的NMDA反应。在有核膜片中，NMDA电流因外部渗透压降低而增强，在高渗溶液中则降低。这些变化在低浓度NMDA时更为明显，而对海人酸、甘氨酸或GABA的反应则未观察到。它们可以通过直接改变有核、外翻、内翻和细胞贴附膜片中的吸管压力来模拟。NMDA反应的渗透压敏感性在全细胞模式下也有观察到，但仅在长时间透析后。NMDA受体的机械敏感性可能在经历膜张力变化的神经元区域（如棘突或生长锥）中起重要作用。
