用于物理图谱绘制的一致性Alu重复序列探针。

A consensus Alu repeat probe for physical mapping.

作者信息

Batzer M A, Alegria-Hartman M, Deininger P L

机构信息

Human Genome Center, Lawrence Livermore National Laboratory, Livermore, California 94551.

出版信息

Genet Anal Tech Appl. 1994;11(2):34-8. doi: 10.1016/1050-3862(94)90058-2.

DOI:10.1016/1050-3862(94)90058-2

PMID:7917498

Abstract

Physical mapping of the human genome involves a variety of complex hybridization-based procedures, some of which rely upon the ability to separate human clones derived from human-rodent hybrid cell lines from those that contain background rodent-derived DNA sequences. The ability to block the repetitive element (Alu repeat) portion of inter-Alu PCR products derived from a variety of complex sources is also crucial for the isolation of unique DNA sequences. Here we report the construction and characterization of a new consensus Alu repeat probe (pPD39) designed for these purposes.

摘要

人类基因组的物理图谱绘制涉及多种基于杂交的复杂程序，其中一些程序依赖于将源自人-鼠杂交细胞系的人类克隆与那些含有背景鼠源DNA序列的克隆区分开来的能力。阻断源自多种复杂来源的Alu间PCR产物的重复元件（Alu重复序列）部分的能力对于分离独特的DNA序列也至关重要。在此，我们报告了为这些目的设计的一种新的共有Alu重复序列探针（pPD39）的构建及特性分析。