Brooks-Wilson A R, Goodfellow P N, Povey S, Nevanlinna H A, de Jong P J, Goodfellow P J
Department of Medical Genetics, University of British Columbia, Vancouver, Canada.
Genomics. 1990 Aug;7(4):614-20. doi: 10.1016/0888-7543(90)90207-b.
Alu element-mediated polymerase chain reaction is a strategy for rapidly cloning and mapping human DNA markers from mixed DNA sources. A novel primer homologous to the 3' end of the human Alu repeat element provides the basis for preferential synthesis of human DNA fragments from human/rodent somatic cell hybrid DNA template. This approach has been used to isolate a series of new markers from chromosome 10. The Alu element-mediated PCR probes were regionally assigned on chromosome 10 by hybridization to Southern blots of Alu PCR-synthesized DNA derived from somatic cell hybrid template DNA. Alu element-mediated PCR is generally applicable and makes possible the analysis of complex genomes with a speed and sensitivity that has not been previously possible.
Alu元件介导的聚合酶链反应是一种从混合DNA来源快速克隆和定位人类DNA标记的策略。一种与人类Alu重复元件3'端同源的新型引物为从人/啮齿动物体细胞杂种DNA模板中优先合成人类DNA片段提供了基础。该方法已用于从10号染色体分离一系列新标记。通过与源自体细胞杂种模板DNA的Alu PCR合成DNA的Southern印迹杂交，将Alu元件介导的PCR探针定位到10号染色体上。Alu元件介导的PCR普遍适用，能够以前所未有的速度和灵敏度分析复杂基因组。
