Sugerman P B, Savage N W, Seymour G J
Department of Dentistry, University of Queensland, St Lucia, Australia.
Br J Dermatol. 1994 Sep;131(3):319-24. doi: 10.1111/j.1365-2133.1994.tb08517.x.
Lymphocytes were extracted from six biopsy specimens of oral lichen planus. T-lymphocyte lines were expanded in culture with phytohaemagglutinin and interleukin 2, and cloned by limiting dilution. Fifteen T-cell clones were isolated with a probability of clonality of 96.3%. The majority of clones (n = 13) expressed the alpha beta T-cell receptor, and of these, 11 were CD8+ and two were CD4+. Two clones were CD4- and CD8-, and expressed the gamma delta T-cell receptor. The ability of these clones (effectors) to suppress concanavalin-A-stimulated proliferation of autologous lesional T-cell lines (responders) was assessed. Maximum suppressor activity ranged from 17 to 100%. The majority of clones (n = 12), including a CD3+ CD4+ CD8-alpha beta+ clone, displayed suppressor activity which was proportional to the effector to responder ratio. A CD3+CD4+CD8-alpha beta+ clone and a CD3+CD4-CD8-gamma delta+ clone displayed substantial helper activity at higher effector to responder ratios. These results demonstrate differential helper and suppressor activity of T-lymphocyte clones extracted from oral lichen planus lesions. The balance between help and suppression may be a fundamental determinant of immunological activity within the lymphocytic infiltrate of oral lichen planus, and hence may dictate the clinical behaviour of the disease.
从6例口腔扁平苔藓活检标本中提取淋巴细胞。用植物血凝素和白细胞介素2在培养中扩增T淋巴细胞系，并通过有限稀释进行克隆。分离出15个T细胞克隆，克隆性概率为96.3%。大多数克隆（n = 13）表达αβT细胞受体，其中11个为CD8 +，2个为CD4 +。2个克隆为CD4 - 和CD8 -，并表达γδT细胞受体。评估了这些克隆（效应细胞）抑制伴刀豆球蛋白A刺激的自体病变T细胞系（反应细胞）增殖的能力。最大抑制活性范围为17%至100%。大多数克隆（n = 12），包括一个CD3 + CD4 + CD8 - αβ +克隆，表现出与效应细胞与反应细胞比例成比例的抑制活性。一个CD3 + CD4 + CD8 - αβ +克隆和一个CD3 + CD4 - CD8 - γδ +克隆在较高的效应细胞与反应细胞比例下表现出显著的辅助活性。这些结果表明从口腔扁平苔藓病变中提取的T淋巴细胞克隆具有不同的辅助和抑制活性。辅助和抑制之间的平衡可能是口腔扁平苔藓淋巴细胞浸润内免疫活性的一个基本决定因素，因此可能决定该疾病的临床行为。
