Fukuda Y, Fujita H, Taketani S, Sassa S
Rockefeller University, New York, N.Y. 10021.
Br J Haematol. 1993 Dec;85(4):670-5. doi: 10.1111/j.1365-2141.1993.tb03207.x.
The level of mRNA encoding ferrochelatase (FeC) was examined in two murine erythroleukaemia (MEL) clones, DS and DR, a DMSO-sensitive, and a DMSO-resistant clone, respectively. DS cells undergo erythroid differentiation by DMSO treatment with a marked increase in haem synthesis, while DR cells fail to do so due to the lack of the erythroid-specific delta-aminolaevulinate synthase (ALAS-E). Both DS and DR cells showed an increase in the level of FeC mRNA within 18 h of DMSO treatment. The level of FeC mRNA in DR cells was then decreased, while that in DS cells continued to increase for 72 h. Treatment with haemin significantly increased FeC mRNA in DR cells. When cells were treated with both DMSO and haemin, the level of FeC mRNA in DR cells increased to a level comparable to that in DS cells. These findings suggest that the failure to maintain increased FeC mRNA DR cells after DMSO treatment may be due to a deficiency of haem in these cells.
分别在两个小鼠红白血病（MEL）克隆DS和DR中检测了编码亚铁螯合酶（FeC）的mRNA水平，DS是对二甲基亚砜（DMSO）敏感的克隆，DR是对DMSO耐药的克隆。DS细胞通过DMSO处理进行红系分化，血红素合成显著增加，而DR细胞由于缺乏红系特异性δ-氨基-γ-酮戊酸合酶（ALAS-E）而无法进行红系分化。DS和DR细胞在DMSO处理18小时内FeC mRNA水平均升高。随后DR细胞中FeC mRNA水平下降，而DS细胞中的FeC mRNA水平持续升高72小时。血红素处理显著增加了DR细胞中的FeC mRNA。当细胞同时用DMSO和血红素处理时，DR细胞中FeC mRNA水平增加到与DS细胞相当的水平。这些发现表明，DMSO处理后DR细胞未能维持FeC mRNA水平升高可能是由于这些细胞中血红素缺乏。
