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The effects of saline solutions on red cell shape: a scanning-electron- microscope-based study.

作者信息

Simpson L O

机构信息

Department of General Practice, University of Otago Medical School, Dunedin, New Zealand.

出版信息

Br J Haematol. 1993 Dec;85(4):832-4. doi: 10.1111/j.1365-2141.1993.tb03237.x.

DOI:10.1111/j.1365-2141.1993.tb03237.x
PMID:7918057
Abstract

Red cells appear to change shape in response to alterations in their environment both in vitro and in vivo. To investigate the qualitative aspects of this phenomenon, five drops of a venous blood sample were fixed in buffered glutaraldehyde for baseline observations and 0.5 ml of blood added to 4 ml of four different saline solutions. Triplicated 10-drop samples from the suspensions were fixed in the glutaraldehyde solution after 2.5, 5, 10, 20 and 40 min and prepared for scanning electron microscopy. Red cell shape analysis of the resulting micrographs showed that the cells had changed shape, although no two patterns of change were the same.

摘要

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