Iwakiri R, Inokuchi K, Dan K, Nomura T
Third Department of Internal Medicine, Nippon Medical School, Tokyo, Japan.
Br J Haematol. 1994 Apr;86(4):870-2. doi: 10.1111/j.1365-2141.1994.tb04845.x.
We report a patient with acute promyelocytic leukaemia who developed marked basophilia during all-trans retinoic acid treatment. We studied genomic DNA and RNA extracted from the patient's peripheral leucocytes in order to determine the origin of the basophils. The RAR alpha rearranged band in the Southern blot analysis and a chimaeric product of PML-RAR alpha by polymerase chain reaction were strongly visible before ATRA treatment, but at the time of maximal basophilia both of them were markedly diminished. These findings suggest that the basophils which appeared during the ATRA treatment are reactive in nature rather than a leukaemic clone.
我们报告了1例急性早幼粒细胞白血病患者，其在全反式维甲酸治疗期间出现明显的嗜碱性粒细胞增多。我们研究了从患者外周血白细胞中提取的基因组DNA和RNA，以确定嗜碱性粒细胞的来源。在全反式维甲酸治疗前，Southern印迹分析中的RARα重排条带和聚合酶链反应检测到的PML-RARα嵌合产物均清晰可见，但在嗜碱性粒细胞增多最明显时，两者均显著减少。这些发现提示，全反式维甲酸治疗期间出现的嗜碱性粒细胞本质上是反应性的，而非白血病克隆。
