González-Gaitán M, Capdevila M P, García-Bellido A
Centro de Biología Molecular, C.S.I.C., Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Mech Dev. 1994 Jun;46(3):183-200. doi: 10.1016/0925-4773(94)90070-1.
Morphogenetic processes, based on the temporal and spatial control of cell proliferation, are involved in determining the size and shape of an organism. We have used clonal analysis, employing X-ray-induced mitotic recombination, to study cell proliferation and differentiation processes in the developing wing imaginal disc of Drosophila. Our results show a non-uniform distribution of mitotic activities during different stages of wing development. This may reflect waves of cell proliferation which derive from distinct centers of cell proliferation within the growing wing imaginal disc. These proliferation centers are located within the major wing compartments (i.e. the anterior, posterior, dorsal and ventral compartments) and they are restricted to the areas which give rise to the intervein regions of the adult wing. The mitotic recombination analysis, combined with the study of Minute and gynandromorph mosaics, show that the presumptive vein regions of the wing represent distinct boundaries which delimit the proliferation centers to the intervein regions. We present a generative model of wing morphogenesis that is consistent with our results.
基于细胞增殖的时空控制的形态发生过程，参与决定生物体的大小和形状。我们利用克隆分析，通过X射线诱导的有丝分裂重组，来研究果蝇发育中的翅成虫盘的细胞增殖和分化过程。我们的结果显示，在翅发育的不同阶段，有丝分裂活动分布不均。这可能反映了源自生长中的翅成虫盘内不同细胞增殖中心的细胞增殖波。这些增殖中心位于主要的翅区室（即前区、后区、背区和腹区）内，并且局限于产生成虫翅翅脉间区域的区域。有丝分裂重组分析，结合对Minute和雌雄嵌合体镶嵌体的研究，表明翅的假定翅脉区域代表了不同的边界，这些边界将增殖中心限定在翅脉间区域。我们提出了一个与我们的结果一致的翅形态发生生成模型。
