Liaubet A, Egret-Charlier M, Kuhry J G
Centre de Biophysique Moléculaire, UPR 4301 du CNRS, Orléans, France.
Biochim Biophys Acta. 1994 Oct 12;1195(1):164-8. doi: 10.1016/0005-2736(94)90023-x.
Endocytic coated vesicles (CV) were purified from bovine brain, and uncoated vesicles (UV) were obtained from the latter by dialysis against 1 M Tris. Membrane dynamics were explored in both vesicle populations using two complementary fluorescence approaches: diphenylhexatriene fluorescence anisotropy to account for rotational lipid movements, and pyrene excimerization with a phosphatidylcholine pyrene derivative for translational motion. It was concluded that membrane fluidity was considerably higher in UV than in CV, and that adding bulk coat proteins (adaptors+clathrin) to uncoated vesicles re-established the low fluidity found in coated vesicles. However, adding coat protein constituents separately had no effect.
