Föhr U G, Gibson G E, Tofel-Grehl B, Schäfer B W, Heizmann C W
Department of Pediatrics, University of Zurich, Switzerland.
Biochim Biophys Acta. 1994 Sep 29;1223(3):391-7. doi: 10.1016/0167-4889(94)90100-7.
Disturbed calcium homeostasis may play a role in the etiology in Alzheimer's and other neurodegenerative diseases. A protective role against cellular degeneration has been postulated for Ca(2+)-binding proteins in certain neuron populations. Recent data suggest that intracellular free calcium regulation is also altered in several non-neuronal cells, including skin fibroblasts, from patients with Alzheimer's disease. In this study we analyzed the expression of several EF-hand Ca(2+)-binding proteins in cultured skin fibroblasts from Alzheimer patients and age-matched normal donors. We detected a strong expression of some members of the S100 Ca(2+)-binding protein family and of calcineurin A. However, no significant differences were found between both types of donors by Northern blot and Western blot analysis. In addition, similar signals were detected on 45Ca(2+)-blots of fibroblasts extracts of Alzheimer patients and control donors. The present findings indicate that the altered level of some intracellular calcium-binding proteins in certain brain areas of Alzheimer patients is not found in skin fibroblasts of these patients.
钙稳态失调可能在阿尔茨海默病和其他神经退行性疾病的病因中起作用。在某些神经元群体中，钙结合蛋白对细胞变性具有保护作用。最近的数据表明，阿尔茨海默病患者的几种非神经元细胞（包括皮肤成纤维细胞）的细胞内游离钙调节也发生了改变。在本研究中，我们分析了阿尔茨海默病患者和年龄匹配的正常供体培养的皮肤成纤维细胞中几种EF手型钙结合蛋白的表达。我们检测到S100钙结合蛋白家族的一些成员和钙调神经磷酸酶A的强烈表达。然而，通过Northern印迹和Western印迹分析，在两种类型的供体之间未发现显著差异。此外，在阿尔茨海默病患者和对照供体的成纤维细胞提取物的45Ca（2+）印迹上检测到相似的信号。目前的研究结果表明，在这些患者的皮肤成纤维细胞中未发现阿尔茨海默病患者某些脑区中一些细胞内钙结合蛋白水平的改变。
