Grondin G, Grondin G G, Talbot B G
Department of Biology, Faculty of Sciences, University of Sherbrooke, Québec, Canada.
Biotech Histochem. 1994 Jul;69(4):219-34. doi: 10.3109/10520299409106291.
Plastination permits the preservation of anatomical specimens in a physical state approaching that of the living condition. We studied the possibility of using silicone plastinated fragments of spleen and pancreas for optical and electron microscopy, and found that with an adequate fixation protocol, plastinated specimens can be used for both light microscopy and ultrastructural studies. Deplastination with sodium methoxide permitted production of clean sections. Artifacts produced by plastination/deplastination could be nearly eliminated by glutaraldehyde/formaldehyde fixation. The (Biodur) silicone S10 polymer is transparent and stable in an electron beam, and plastinated tissues can be contrasted or colored similar to tissues embedded in Epon 812. In addition to being very life-like, plastinated tissues are stable and easy to handle. They can also be used for electron and light microscopic studies. This technique may also allow retrospective epidemiological studies of archived pathology specimens.
塑化技术能够将解剖标本保存在接近活体状态的物理状态。我们研究了使用脾脏和胰腺的硅基塑化切片进行光学显微镜和电子显微镜观察的可能性，发现通过适当的固定方案，塑化标本可用于光学显微镜和超微结构研究。用甲醇钠进行脱塑化处理可制作出清晰的切片。通过戊二醛/甲醛固定，几乎可以消除塑化/脱塑化过程中产生的伪像。（Biodur）硅基S10聚合物在电子束下是透明且稳定的，并且塑化组织可以像包埋在Epon 812中的组织一样进行对比或染色。除了非常逼真外，塑化组织稳定且易于处理。它们还可用于电子显微镜和光学显微镜研究。这项技术也可能允许对存档的病理标本进行回顾性流行病学研究。
