Simpson J N, McGinty J F
Department of Anatomy and Cell Biology, East Carolina University School of Medicine, Greenville, NC 27858-4354.
Neuroreport. 1994 Jun 2;5(10):1213-6. doi: 10.1097/00001756-199406020-00013.
Promoter regions of the preproenkephalin, preprodynorphin, and c-fos genes contain cyclase response elements (CREs) as well as AP-1 sites. Activation of the adenylate cyclase cascade leads to phosphorylation of cyclase response element binding proteins (P-CREBs) which then bind CREs in these genes and induce transcription. In this experiment, semi-quantitative immunocytochemistry was used to examine striatal CREB-, P-CREB-, and Fos-like immunoreactivity (IR) 1 h following intracerebroventricular injection of H2O-soluble forskolin. Although forskolin did not alter CREB-IR, forskolin did induce striatal P-CREB-IR and Fos-IR by 2.5- and 10-fold, respectively. These data support a role for P-CREB and/or Fos in regulating opioid peptide gene transcription following direct in vivo activation of adenylate cyclase.
前脑啡肽原、前强啡肽原和c-fos基因的启动子区域包含环化酶反应元件（CREs）以及AP-1位点。腺苷酸环化酶级联反应的激活导致环化酶反应元件结合蛋白（P-CREBs）磷酸化，然后P-CREBs与这些基因中的CREs结合并诱导转录。在本实验中，采用半定量免疫细胞化学方法检测脑室内注射水溶性福司可林1小时后纹状体中CREB、P-CREB和Fos样免疫反应性（IR）。尽管福司可林未改变CREB-IR，但它分别使纹状体P-CREB-IR和Fos-IR诱导增加了2.5倍和10倍。这些数据支持P-CREB和/或Fos在体内直接激活腺苷酸环化酶后调节阿片肽基因转录中的作用。
