Banati R B, Gehrmann J, Kreutzberg G W
Department of Neuromorphology, Max-Planck-Institute for Psychiatry, Martinsried, Germany.
Neuroreport. 1994 Jun 27;5(11):1359-61. doi: 10.1097/00001756-199406270-00016.
Stereotactic lesioning of the rat entorhinal cortex leads to an induction of beta-amyloid precursor protein (APP) immunoreactivity in non-neuronal cells of the deafferented dentate gyrus. Double immunofluorescence against APP and the microglia-binding isolectin B4 from Griffonia simplicifolia revealed that APP immunoreactivity was confined to activated microglia. The microglial APP expression became detectable 3 days after lesioning, reached its peak after 7 days and disappeared after 10 days. The early accumulation of APP in microglia supports the view that microglia play an important role in the initial stages of amyloid plaque formation. Such a glial APP accumulation occurs rapidly in distant but anatomically connected areas. This is in line with the preferential localization of amyloid plaques in the dentate gyrus, which is the projection field of the degenerating neurones of the entorhinal cortex in patients with developing dementia.
对大鼠内嗅皮质进行立体定向损伤会导致去传入齿状回的非神经元细胞中β-淀粉样前体蛋白（APP）免疫反应性增强。针对APP和来自非洲相思树的小胶质细胞结合异凝集素B4进行双重免疫荧光检测发现，APP免疫反应性局限于活化的小胶质细胞。损伤后3天可检测到小胶质细胞APP表达，7天后达到峰值，10天后消失。APP在小胶质细胞中的早期积累支持了小胶质细胞在淀粉样斑块形成初始阶段起重要作用的观点。这种胶质细胞APP积累在远处但解剖学上相连的区域迅速发生。这与淀粉样斑块在齿状回中的优先定位一致，齿状回是患有进行性痴呆患者内嗅皮质退化神经元的投射区域。
